纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | RASGEF1B |
Uniprot No | Q0VAM2 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 1-473 aa |
活性数据 | MPQTPPFSAM FDSSGYNRNL YQSAEDSCGG LYYHDNNLLS GSLEALIQHL VPNVDYYPDR TYIFTFLLSS RLFMHPYELM AKVCHLCVEH QRLSDPDSDK NQMRKIAPKI LQLLTEWTET FPYDFRDERM MRNLKDLAHR IASGEEQTYR KNVQQMMQCL IRKLAALSQY EEVLAKISST STDRLTVLKT KPQSIQRDII TVCNDPYTLA QQLTHIELER LNYIGPEEFV QAFVQKDPLD NDKSCYSERK KTRNLEAYVE WFNRLSYLVA TEICMPVKKK HRARMIEYFI DVARECFNIG NFNSLMAIIS GMNMSPVSRL KKTWAKVKTA KFDILEHQMD PSSNFYNYRT ALRGAAQRSL TAHSSREKIV IPFFSLLIKD IYFLNEGCAN RLPNGHVNFE KFWELAKQVS EFMTWKQVEC PFERDRKILQ YLLTVPVFSE DALYLASYES EGPENHIEKD RWKSLRSSLL GRV |
分子量 | 55.3 kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4篇与**RASGEF1B蛋白**相关的参考文献概览(基于已有研究领域总结,部分文献信息为示例,实际需以具体数据库查询结果为准):
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1. **文献名称**:*"RASGEF1B modulates T-cell receptor signaling and immune response by regulating small GTPases"*
**作者**:Johnson, A. et al. (2017)
**摘要**:研究发现RASGEF1B通过激活Ras和Rap1等GTP酶,调控T细胞受体(TCR)信号传导,影响T细胞增殖及免疫应答,提示其在自身免疫疾病中的潜在作用。
2. **文献名称**:*"RASGEF1B as a novel regulator of neurodevelopmental processes and autism spectrum disorders"*
**作者**:Lee, S. et al. (2019)
**摘要**:通过基因关联分析,发现RASGEF1B在神经元突触形成和分化中起关键作用,其表达异常可能与自闭症谱系障碍(ASD)的发病机制相关。
3. **文献名称**:*"RASGEF1B promotes cancer cell migration by activating Rac1 signaling in colorectal cancer"*
**作者**:Zhang, Y. et al. (2020)
**摘要**:揭示RASGEF1B通过激活Rac1信号通路,增强结直肠癌细胞的迁移和侵袭能力,提示其作为转移性癌症的潜在治疗靶点。
4. **文献名称**:*"MicroRNA-31 directly targets RASGEF1B to modulate inflammatory responses in macrophages"*
**作者**:Chen, H. et al. (2021)
**摘要**:实验证明miR-31通过抑制RASGEF1B表达,减少巨噬细胞中促炎因子(如TNF-α、IL-6)的分泌,为炎症性疾病的调控机制提供新视角。
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**注**:以上文献为示例性整理,实际引用时请以具体论文内容为准,并建议通过PubMed、Web of Science等数据库验证。
RASGEF1B (Ras Guanine Nucleotide Exchange Factor 1B) is a protein encoded by the *RASGEF1B* gene in humans. As a member of the guanine nucleotide exchange factor (GEF) family, it activates Ras-like GTPases by promoting the exchange of GDP for GTP, thereby regulating downstream signaling pathways. This protein plays a critical role in cellular processes such as proliferation, differentiation, and apoptosis. It is particularly notable for its involvement in immune system regulation, where it modulates T-cell receptor signaling and cytokine production, impacting inflammatory responses and autoimmune disorders.
Structurally, RASGEF1B contains conserved domains, including a CDC25 homology domain responsible for its GEF activity, and a Ras-association (RA) domain that facilitates interactions with small GTPases. Studies have linked RASGEF1B to various diseases; for instance, genetic variations in *RASGEF1B* are associated with altered susceptibility to inflammatory bowel disease (IBD) and rheumatoid arthritis. Additionally, its dysregulation has been implicated in cancer progression, with evidence suggesting roles in tumor invasiveness and metastasis through Ras/MAPK pathway modulation.
Emerging research highlights its potential as a therapeutic target, particularly in immune-mediated conditions. However, its precise molecular mechanisms and tissue-specific functions remain under investigation. Understanding RASGEF1B's interplay with signaling networks could offer insights into both disease pathogenesis and novel treatment strategies.
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