| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RASGRP3 |
| Uniprot No | Q8IV61 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-690 aa |
| 活性数据 | MGSSGLGKAATLDELLCTCIEMFDDNGELDNSYLPRIVLLMHRWYLSSTELAEKLLCMYRNATGESCNEFRLKICYFMRYWILKFPAEFNLDLGLIRMTEEFREVASQLGYEKHVSLIDISSIPSYDWMRRVTQRKKVSKKGKACLLFDHLEPIELAEHLTFLEHKSFRRISFTDYQSYVIHGCLENNPTLERSIALFNGISKWVQLMVLSKPTPQQRAEVITKFINVAKKLLQLKNFNTLMAVVGGLSHSSISRLKETHSHLSSEVTKNWNEMTELVSSNGNYCNYRKAFADCDGFKIPILGVHLKDLIAVHVIFPDWTEENKVNIVKMHQLSVTLSELVSLQNASHHLEPNMDLINLLTLSLDLYHTEDDIYKLSLVLEPRNSKSQPTSPTTPNKPVVPLEWALGVMPKPDPTVINKHIRKLVESVFRNYDHDHDGYISQEDFESIAANFPFLDSFCVLDKDQDGLISKDEMMAYFLRAKSQLHCKMGPGFIHNFQEMTYLKPTFCEHCAGFLWGIIKQGYKCKDCGANCHKQCKDLLVLACRRFARAPSLSSGHGSLPGSPSLPPAQDEVFEFPGVTAGHRDLDSRAITLVTGSSRKISVRLQRATTSQATQTEPVWSEAGWGDSGSHTFPKMKSKFHDKAAKDKGFAKWENEKPRVHAGVDVVDRGTEFELDQDEGEETRQDGEDG |
| 分子量 | 85.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RASGRP3蛋白的3篇参考文献示例，涵盖其在信号通路、疾病机制及结构解析中的研究：
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1. **标题**: *RASGRP3 regulates B cell receptor-induced activation of ERK in B cells*
**作者**: V.J. Lawson, D.L. Hsu, et al. (2016)
**摘要**: 研究揭示了RASGRP3在B细胞受体信号传导中的作用，通过激活RAS-ERK通路调控B细胞响应抗原刺激，基因敲除实验显示该蛋白缺失会显著抑制ERK磷酸化及细胞增殖。
2. **标题**: *RASGRP3 overexpression promotes melanoma progression via MAPK pathway activation*
**作者**: M.J. Rieder, T. Kurosawa, et al. (2018)
**摘要**: 在黑色素瘤中发现RASGRP3的高表达通过激活MAPK通路驱动肿瘤生长和转移，体外和小鼠模型实验表明抑制RASGRP3可减少癌细胞侵袭，提示其作为潜在治疗靶点。
3. **标题**: *Structural basis of RASGRP3 recognition by protein kinase C*
**作者**: J. Zhang, R. Li, et al. (2020)
**摘要**: 通过X射线晶体学解析RASGRP3与PKC的复合物结构，阐明两者结合的关键结构域，为设计靶向RASGRP3-PKC相互作用的药物提供理论依据。
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**注**：上述文献为示例，实际引用时请核实数据库(如PubMed、Google Scholar)中的最新研究，并补充完整期刊名称、卷号及页码。若需具体文献，建议以“RASGRP3”和“recombinant protein”为关键词进一步检索。
RASGRP3 (RAS Guanyl Releasing Protein 3) is a member of the RASGRP family of guanine nucleotide exchange factors (GEFs) that activate RAS and RAP1 GTPases by catalyzing the exchange of GDP for GTP. Structurally, it contains critical domains including an N-terminal EF-hand motif for calcium binding, a central REM (RAS exchange motif) domain, a RAS-association (RA) domain, and a C-terminal catalytic CDC25 homology domain. Expressed in immune cells, liver, and neural tissues, RASGRP3 plays pivotal roles in signaling pathways regulating cell proliferation, differentiation, and survival.
In immune regulation, RASGRP3 modulates B-cell receptor (BCR) signaling and T-cell responses by integrating calcium and diacylglycerol (DAG) signals to activate downstream MAPK/ERK pathways. Dysregulation of RASGRP3 is linked to autoimmune diseases, such as lupus, and cancers. For instance, its overexpression in melanoma and leukemia enhances RAS/MAPK signaling, promoting oncogenesis and therapy resistance. Additionally, RASGRP3 participates in metabolic processes, influencing hepatic insulin sensitivity via RAP1-dependent pathways. Recent studies highlight its context-dependent roles, where it can either suppress or promote tumor growth depending on cellular environment and interacting partners. Its dual regulation of RAS and RAP1 adds complexity to its functional characterization, making RASGRP3 a compelling target for therapeutic strategies in cancer and immune disorders. Research continues to unravel its tissue-specific signaling mechanisms and potential as a biomarker.
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