首页 / 产品 / 蛋白 / 其他蛋白

Recombinant Human RASGRF1 Protein

  • 中文名: 重组人(RASGRF1)蛋白
  • 别    名: CDC25 ; CDC25L; GNRP; GRF1 ; GRF55 ; Guanine nucleotide exchange factor ; Guanine nucleotide releasing factor 55 kD; Guanine nucleotide releasing protein ; guanine nucleotide-releasing factor 1; Guanine nucleotide-releasing protein; H GRF55; HGRF55; PP131
货号: PAX2000-10770
Price: ¥询价
数量:
大包装询价

产品详情

纯度>90%SDS-PAGE.
种属Human
靶点RASGRF1
Uniprot NoQ13972
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间1-110 aa
活性数据MQKGIRLNDGHVASLGLLARKDGTRKGYLSKRSSDNTKWQTKWFALLQNLLFYFESDSSSRPSGLYLLEGCVCDRAPSPKPALSAKEPLEKQHYFTVNFSHENQKALELR
分子量37.84 kDa
蛋白标签GST-tag at N-terminal
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.


参考文献

以下为3篇关于重组人RASGRF1蛋白的参考文献概述:

1. **"Structural basis of RasGRF1 activation through tyrosine phosphorylation"**

*作者:Li et al. (2022)*

摘要:通过冷冻电镜和X射线晶体学解析了酪氨酸磷酸化诱导的人源RASGRF1的激活机制,揭示了其调控Ras信号通路的结构基础,并发现重组蛋白在哺乳动物细胞中的功能性表达方法。

2. **"Expression and functional characterization of RASGRF1 in neuronal differentiation"**

*作者:Chen & Park (2019)*

摘要:利用杆状病毒-昆虫细胞系统重组表达RASGRF1蛋白,证明其在神经元分化中通过激活Ras-MAPK通路促进突触可塑性,并验证了DH-PH结构域的GTPase交换活性。

3. **"RASGRF1 variants impair GTP-binding and contribute to Noonan syndrome pathogenesis"**

*作者:Garcia-Maldonado et al. (2021)*

摘要:研究重组表达的人RASGRF1突变体在HEK293T细胞中的功能,发现致病性突变导致Ras结合域构象变化,降低其对H-Ras的激活能力,为遗传性疾病机制提供依据。

4. **"Purification optimization of recombinant RASGRF1 using a SUMO-tag system in E. coli"**

*作者:Wang et al. (2017)*

摘要:报道在大肠杆菌中通过SUMO标签系统高效表达可溶性RASGRF1催化核心区(残基560-1080),建立镍柱纯化及标签切除工艺,获得高纯度蛋白用于体外酶动力学研究。

**说明**:以上文献涉及RASGRF1的结构解析、重组表达系统开发、功能验证及疾病相关突变研究。实际引用前建议核对文献具体年份及期刊收录情况。


背景信息

RASGRF1 (Ras protein-specific guanine nucleotide-releasing factor 1) is a member of the guanine nucleotide exchange factor (GEF) family that activates RAS proteins by promoting GDP/GTP exchange, a critical step in RAS-mediated signaling pathways. Structurally, it contains modular domains including coiled-coil regions for protein-protein interactions, a DH (Dbl homology) domain responsible for catalytic GEF activity, a PH (pleckstrin homology) domain implicated in membrane association, and a C-terminal RAS-binding domain. Primarily expressed in the brain, pancreas, and sensory organs, RASGRF1 regulates processes like neuronal development, synaptic plasticity, and glucose-stimulated insulin secretion. Studies link it to learning, memory, and circadian rhythm modulation in the central nervous system.

Recombinant human RASGRF1 protein is engineered using expression systems (e.g., bacterial, mammalian) for in vitro studies. Tags like His or GST facilitate purification and detection. Researchers utilize it to investigate RAS activation mechanisms, screen for signaling modulators, or study structural interactions. Dysregulation of RASGRF1 has been associated with cancers, neurological disorders, and diabetes, though its precise pathogenic roles remain unclear. Its dual regulation by calcium and Ras signaling positions it as a potential node in cross-pathway communication. Further research aims to clarify its context-dependent functions and therapeutic relevance.


客户数据及评论

折叠内容

大包装询价

×