| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RASGEF1A |
| Uniprot No | Q8N9B8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-481 aa |
| 活性数据 | MPQTSVVFSS ILGPSCSGQV QPGMGERGGG AGGGSGDLIF QDGHLISGSL EALMEHLVPT VDYYPDRTYI FTFLLSSRVF MPPHDLLARV GQICVEQKQQ LEAGPEKAKL KSFSAKIVQL LKEWTEAFPY DFQDEKAMAE LKAITHRVTQ CDEENGTVKK AIAQMTQSLL LSLAARSQLQ ELREKLRPPA VDKGPILKTK PPAAQKDILG VCCDPLVLAQ QLTHIELDRV SSIYPEDLMQ IVSHMDSLDN HRCRGDLTKT YSLEAYDNWF NCLSMLVATE VCRVVKKKHR TRMLEFFIDV ARECFNIGNF NSMMAIISGM NLSPVARLKK TWSKVKTAKF DVLEHHMDPS SNFCNYRTAL QGATQRSQMA NSSREKIVIP VFNLFVKDIY FLHKIHTNHL PNGHINFKKF WEISRQIHEF MTWTQVECPF EKDKKIQSYL LTAPIYSEEA LFVASFESEG PENHMEKDSW KTLRTTLLNR A |
| 分子量 | 54.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RASGEF1A蛋白的3篇代表性参考文献示例(内容基于已知研究领域概括，建议通过文献数据库核对完整信息)：
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1. **文献名称**：*"RASGEF1A modulates Ras/MAPK signaling during neuronal development"*
**作者**：Thompson et al.
**摘要**：研究发现RASGEF1A通过激活Ras信号通路参与神经元突触的形成和轴突导向。敲低RASGEF1A会导致小鼠皮层神经元迁移缺陷，提示其在神经发育中的关键作用。
2. **文献名称**：*"Structural and functional characterization of RASGEF1A in cancer cell proliferation"*
**作者**：Chen et al.
**摘要**：解析RASGEF1A的晶体结构，揭示其通过结合Ras-GDP促进核苷酸交换的分子机制。体外实验表明，RASGEF1A过表达显著增强结直肠癌细胞的增殖和侵袭能力。
3. **文献名称**：*"RASGEF1A as a novel biomarker in heart failure pathogenesis"*
**作者**：Rodríguez et al.
**摘要**：通过基因表达分析发现，RASGEF1A在心力衰竭患者心肌组织中表达异常，调控心肌细胞的凋亡和纤维化过程，可能成为治疗靶点。
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**说明**：以上为基于RASGEF1A已知功能的概括性示例，实际文献需通过PubMed或Google Scholar等平台检索确认。RASGEF1A的研究多聚焦于其作为鸟苷酸交换因子(GEF)对Ras/MAPK通路的调控，涉及神经、癌症及心血管疾病等领域。
RASGEF1A (RasGEF Domain-Containing Family Member 1A) is a protein encoded by the RASGEF1A gene, belonging to the guanine nucleotide exchange factor (GEF) family. GEFs regulate small GTPases like Ras by promoting the exchange of GDP for GTP, a critical step in activating downstream signaling pathways involved in cell proliferation, differentiation, and survival. RASGEF1A specifically contains a conserved RasGEF catalytic domain, which facilitates its interaction with Ras-family GTPases. Unlike other RasGEFs, RASGEF1A is implicated in unique biological contexts, including neuronal development and immune responses. Studies suggest its role in axonal guidance and synaptic plasticity, potentially through modulation of Rap1 or other Ras-related proteins.
Structurally, RASGEF1A features additional domains, such as coiled-coil regions, which may mediate protein-protein interactions or subcellular localization. Its expression is tissue-specific, with higher levels observed in the brain and immune cells. Dysregulation of RASGEF1A has been linked to neurodevelopmental disorders and autoimmune conditions, though mechanistic insights remain limited. Notably, it may act as a scaffold protein, integrating signals from membrane receptors to Ras GTPase networks. Despite its emerging significance, RASGEF1A’s precise molecular targets and regulatory mechanisms require further exploration. Ongoing research aims to elucidate its contributions to disease pathogenesis and therapeutic potential.
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