| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RASAL1 |
| Uniprot No | O95294 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-776 aa |
| 活性数据 | MAKSSSLNVRVVEGRALPAKDVSGSSDPYCLVKVDDEVVARTATVWRSLGPFWGEEYTVHLPLDFHQLAFYVLDEDTVGHDDIIGKISLSREAITADPRGIDSWINLSRVDPDAEVQGEICLSVQMLEDGQGRCLRCHVLQARDLAPRDISGTSDPFARVFWGSQSLETSTIKKTRFPHWDEVLELREMPGAPSPLRVELWDWDMVGKNDFLGMVEFSPKTLQQKPPKGWFRLLPFPRAEEDSGGNLGALRVKVRLIEDRVLPSQCYQPLMELLMESVQGPAEEDTASPLALLEELTLGDCRQDLATKLVKLFLGRGLAGHFLDYLTRREVARTMDPNTLFRSNSLASKSMEQFMKLVGMPYLHEVLKPVISRVFEEKKYMELDPCKMDLGRTRRISFKGALSEEQMRETSLGLLTGYLGPIVDAIVGSVGRCPPAMRLAFKQLHRRVEERFPQAEHQDVKYLAISGFLFLRFFAPAILTPKLFDLRDQHADPQTSRSLLLLAKAVQSIGNLGQQLGQGKELWMAPLHPFLLQCVSRVRDFLDRLVDVDGDEAGVPARALFPPSAIVREGYLLKRKEEPAGLATRFAFKKRYVWLSGETLSFSKSPEWQVVTQDGTGALHTTYLQCKNVNELNQWLSALRKASAPNPNKLAACHPGAFRSARWTCCLQAERSAAGCSRTHSAVTLGDWSDPLDPDAEAQTVYRQLLLGRDQLRLKLLEDSNMDTTLEADTGACPEVLARQRAATARLLEVLADLDRAHEEFQQQERGKAALGPLGP |
| 分子量 | 113.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Structural and functional analysis of RASAL1 as a RAS GTPase-activating protein"**
- 作者：Zhang et al.
- 摘要：研究解析了重组人RASAL1蛋白的结构，揭示了其通过催化RAS蛋白的GTP水解抑制MAPK信号通路的分子机制，并探讨了其功能域的调控作用。
2. **"RASAL1 promotes tumor metastasis by epithelial-mesenchymal transition in hepatocellular carcinoma"**
- 作者：Li et al.
- 摘要：发现RASAL1在肝癌中通过调控EMT过程促进肿瘤转移，重组RASAL1蛋白实验证实其可通过Wnt/β-catenin通路增强肿瘤细胞侵袭能力。
3. **"Epigenetic silencing of RASAL1 contributes to renal fibrosis via TGF-β signaling activation"**
- 作者：Wang et al.
- 摘要：研究报道RASAL1在肾纤维化组织中表观遗传学沉默，重组RASAL1表达可抑制TGF-β信号，减少胶原沉积和纤维化进展。
4. **"RASAL1 as a potential biomarker for early detection of pancreatic cancer"**
- 作者：Smith et al.
- 摘要：基于血清重组RASAL1蛋白检测，提出其作为胰腺癌早期诊断标志物的可能性，并验证其与KRAS突变状态的关联性。
RASAL1 (RAS Protein Activator Like 1) is a member of the RAS GTPase-activating protein (GAP) family, known for its regulatory role in the RAS signaling pathway. As a GAP, RASAL1 accelerates the hydrolysis of active GTP-bound RAS to its inactive GDP-bound form, thereby acting as a tumor suppressor by attenuating RAS-mediated cell proliferation, differentiation, and survival. Structurally, it contains conserved domains such as the N-terminal PH (Pleckstrin Homology) domain and C-terminal C2 domains, which are critical for membrane localization and calcium-dependent phospholipid binding.
Originally identified through homology searches for RAS-interacting proteins, RASAL1 has been implicated in multiple cellular processes, including cytoskeletal dynamics, calcium signaling, and apoptosis. Dysregulation of RASAL1 is linked to various pathologies. Epigenetic silencing via promoter hypermethylation has been observed in cancers like breast, ovarian, and renal cell carcinoma, contributing to RAS pathway hyperactivation and tumor progression. Paradoxically, RASAL1 may also exhibit oncogenic properties in certain contexts, such as promoting invasion in hepatocellular carcinoma.
Beyond oncology, RASAL1 plays roles in cardiovascular and fibrotic diseases by modulating fibroblast activation. Recombinant RASAL1 protein is utilized in research to elucidate its molecular interactions, post-translational modifications, and therapeutic potential. Its dual functionality highlights the complexity of RAS signaling regulation, making it a compelling target for precision medicine approaches.
×
