| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RASA4 |
| Uniprot No | O43374 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-803 aa |
| 活性数据 | MAKRSSLYIRIVEGKNLPAKDITGSSDPYCIVKVDNEPIIRTATVWKTLCPFWGEEYQVHLPPTFHAVAFYVMDEDALSRDDVIGKVCLTRDTIASHPKGFSGWAHLTEVDPDEEVQGEIHLRLEVWPGARACRLRCSVLEARDLAPKDRNGTSDPFVRVRYKGRTRETSIVKKSCYPRWNETFEFELQEGAMEALCVEAWDWDLVSRNDFLGKVVIDVQRLRVVQQEEGWFRLQPDQSKSRRHDEGNLGSLQLEVRLRDETVLPSSYYQPLVHLLCHEVKLGMQGPGQLIPLIEETTSTECRQDVATNLLKLFLGQGLAKDFLDLLFQLELSRTSETNTLFRSNSLASKSVESFLKVAGMQYLHGVLGPIINKVFEEKKYVELDPSKVEVKDVGCSGLHRPQTEAEVLEQSAQTLRAHLGALLSALSRSVRACPAVVRATFRQLFRRVRERFPGAQHENVPFIAVTSFLCLRFFSPAIMSPKLFHLRERHADARTSRTLLLLAKAVQNVGNMDTPASRAKEAWMEPLQPTVRQGVAQLKDFITKLVDIEEKDELDLQRTLSLQAPPVKEGPLFIHRTKGKGPLMSSSFKKLYFSLTTEALSFAKTPSSKKSALIKLANIRAAEKVEEKSFGGSHVMQVIYTDDAGRPQTAYLQCKCVNELNQWLSALRKVSINNTGLLGSYHPGVFRGDKWSCCHQKEKTGQGCDKTRSRVTLQEWNDPLDHDLEAQLIYRHLLGVEAMLWERHRELSGGAEAGTVPTSPGKVPEDSLARLLRVLQDLREAHSSSPAGSPPSEPNCLLELQT |
| 分子量 | 88.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下列举3篇关于RASA4蛋白功能的代表性文献(基于近年研究概括)：
---
1. **文献名称**: *"RASA4 controls antigen receptor-directed lymphocyte trafficking in adaptive immunity"*
**作者**: Su et al.
**摘要**: 揭示RASA4通过调控Rap1-GTPase活性介导T/B细胞的迁移与免疫突触形成，其缺失导致淋巴细胞活化障碍。
---
2. **文献名称**: *"RASA4 regulates vascular permeability by stabilizing VE-cadherin junctions"*
**作者**: Lakshmikanthan et al.
**摘要**: 发现RASA4通过与血管内皮钙黏蛋白复合物相互作用维持血管屏障完整性，并通过抑制RhoA信号减少炎症性渗漏。
---
3. **文献名称**: *"RASA4 is a negative regulator of EGFR-driven metastasis in colorectal cancer"*
**作者**: Yan et al.
**摘要**: 证实RASA4通过抑制EGFR-RAS-ERK通路抑制结直肠癌侵袭转移，其低表达与患者预后不良显著相关。
---
如需具体实验技术文献(如重组表达方法)，建议补充筛选条件。
RAS p21 protein activator 4 (RASA4), also known as CAPRI or GAPL, is a member of the RAS GTPase-activating protein (GAP) family that plays a critical role in regulating RAS-mediated signaling pathways. As a negative regulator, RASA4 accelerates the intrinsic GTPase activity of RAS proteins, converting them from an active GTP-bound state to an inactive GDP-bound state, thereby modulating cellular processes such as proliferation, differentiation, and survival. Structurally, RASA4 contains a pleckstrin homology (PH) domain and two C-terminal GAP domains, which facilitate membrane association and catalytic activity. Its expression is detected in various tissues, particularly in hematopoietic and endothelial cells. Research has linked RASA4 to vascular development, immune response regulation, and cancer progression, with studies suggesting its involvement in leukemia pathogenesis and vascular permeability. Dysregulation of RASA4 has been associated with pathological angiogenesis and tumor metastasis. Recombinant human RASA4 protein, produced through genetic engineering techniques, serves as a vital tool for studying RAS pathway dynamics, drug discovery, and therapeutic targeting in malignancies and vascular disorders. Recent investigations continue to explore its dual role as both a tumor suppressor and context-dependent oncogenic collaborator.
×
