| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAPGEFL1 |
| Uniprot No | Q9UHV5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-662 aa |
| 活性数据 | MKPLEKFLKK QTSQLAGRTV AGGPGGGLGS CGGPGGGGGP GGGGGPAGGQ RSLQRRQSVS RLLLPAFLRE PPAEPGLEPP VPEEGGEPAG VAEEPGSGGP CWLQLEEVPG PGPLGGGGPL RSPSSYSSDE LSPGEPLTSP PWAPLGAPER PEHLLNRVLE RLAGGATRDS AASDILLDDI VLTHSLFLPT EKFLQELHQY FVRAGGMEGP EGLGRKQACL AMLLHFLDTY QGLLQEEEGA GHIIKDLYLL IMKDESLYQG LREDTLRLHQ LVETVELKIP EENQPPSKQV KPLFRHFRRI DSCLQTRVAF RGSDEIFCRV YMPDHSYVTI RSRLSASVQD ILGSVTEKLQ YSEEPAGRED SLILVAVSSS GEKVLLQPTE DCVFTALGIN SHLFACTRDS YEALVPLPEE IQVSPGDTEI HRVEPEDVAN HLTAFHWELF RCVHELEFVD YVFHGERGRR ETANLELLLQ RCSEVTHWVA TEVLLCEAPG KRAQLLKKFI KIAALCKQNQ DLLSFYAVVM GLDNAAVSRL RLTWEKLPGK FKNLFRKFEN LTDPCRNHKS YREVISKMKP PVIPFVPLIL KDLTFLHEGS KTLVDGLVNI EKLHSVAEKV RTIRKYRSRP LCLDMEASPN HLQTKAYVRQ FQVIDNQNLL FELSYKLEAN SQ |
| 分子量 | 73.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RAPGEFL1蛋白的3篇参考文献的简要整理(注：文献信息为假设示例，实际需根据真实数据库检索验证)：
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1. **文献名称**: *RAPGEFL1 regulates renal development via cAMP signaling*
**作者**: Mochizuki, K.A., et al.
**摘要**: 研究发现RAPGEFL1通过介导cAMP信号通路激活下游Rap1.调控小鼠肾脏发育过程中上皮细胞的极性形成和管腔结构生成。重组RAPGEFL1蛋白在体外实验中表现出与EPAC家族蛋白相似的功能。
2. **文献名称**: *Structural insights into RAPGEFL1 and Rap1 interaction*
**作者**: Scholten, R.J., et al.
**摘要**: 通过X射线晶体学解析重组人RAPGEFL1蛋白的结构，揭示了其与Rap1结合的活性域关键位点。该研究证明RAPGEFL1的催化结构域通过构象变化激活Rap1的GTP酶活性，为相关信号通路机制提供了分子基础。
3. **文献名称**: *RAPGEFL1 overexpression promotes cancer cell adhesion via Rap1*
**作者**: Boerner, L.S., et al.
**摘要**: 重组RAPGEFL1在乳腺癌细胞中的过表达实验表明，其通过增强Rap1依赖性整合素信号通路，促进肿瘤细胞与细胞外基质的粘附，可能影响癌症转移的早期步骤。
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**建议**：实际研究中请通过PubMed或Web of Science以“RAPGEFL1”、“recombinant protein”或“Rap1 signaling”为关键词检索最新文献，并核实作者及期刊信息。部分研究可能侧重功能机制而非重组蛋白本身，建议拓展查阅相关信号通路文献。
RAPGEFL1 (RAP Guanine Nucleotide Exchange Factor Like 1), also known as MR-GEF or RA-GEF-2. is a member of the RAS-associated protein (RAP) guanine nucleotide exchange factor (GEF) family. It plays a regulatory role in intracellular signaling by activating RAP GTPases, which are pivotal in processes like cell adhesion, cytoskeletal reorganization, and vesicle trafficking. Structurally, RAPGEFL1 contains conserved domains such as a Ras-association (RA) domain and a catalytic GEF domain, enabling its interaction with small GTPases and facilitating GDP-to-GTP exchange.
The protein is particularly noted for its role in neuroendocrine and hormonal signaling. Studies link RAPGEFL1 to cAMP-mediated signaling pathways, where it interacts with effectors like EPAC (Exchange Protein Activated by cAMP), modulating cellular responses to hormones like insulin and glucagon. Dysregulation of RAPGEFL1 has been implicated in metabolic disorders and neurological conditions, though its mechanistic contributions remain under investigation.
Recombinant RAPGEFL1 protein is typically produced using bacterial or mammalian expression systems, retaining functional domains for in vitro studies. It serves as a tool to dissect RAP GTPase activation mechanisms, screen therapeutic compounds targeting GEF-dependent pathways, and explore crosstalk between cAMP and GTPase signaling. Further research aims to clarify its tissue-specific roles and potential as a biomarker or therapeutic target.
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