| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAP80 |
| Uniprot No | Q96RL1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-719 aa |
| 活性数据 | MPRRKKKVKE VSESRNLEKK DVETTSSVSV KRKRRLEDAF IVISDSDGEE PKEENGLQKT KTKQSNRAKC LAKRKIAQMT EEEQFALALK MSEQEAREVN SQEEEEEELL RKAIAESLNS CRPSDASATR SRPLATGPSS QSHQEKTTDS GLTEGIWQLV PPSLFKGSHI SQGNEAEERE EPWDHTEKTE EEPVSGSSGS WDQSSQPVFE NVNVKSFDRC TGHSAEHTQC GKPQESTGRG SAFLKAVQGS GDTSRHCLPT LADAKGLQDT GGTVNYFWGI PFCPDGVDPN QYTKVILCQL EVYQKSLKMA QRQLLNKKGF GEPVLPRPPS LIQNECGQGE QASEKNECIS EDMGDEDKEE RQESRASDWH SKTKDFQESS IKSLKEKLLL EEEPTTSHGQ SSQGIVEETS EEGNSVPASQ SVAALTSKRS LVLMPESSAE EITVCPETQL SSSETFDLER EVSPGSRDIL DGVRIIMADK EVGNKEDAEK EVAISTFSSS NQVSCPLCDQ CFPPTKIERH AMYCNGLMEE DTVLTRRQKE AKTKSDSGTA AQTSLDIDKN EKCYLCKSLV PFREYQCHVD SCLQLAKADQ GDGPEGSGRA CSTVEGKWQQ RLKNPKEKGH SEGRLLSFLE QSEHKTSDAD IKSSETGAFR VPSPGMEEAG CSREMQSSFT RRDLNESPVK SFVSISEATD CLVDFKKQVT VQPGSRTRTK AGRGRRRKF |
| 分子量 | 79.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RAP80蛋白的3篇关键文献的简要列举：
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1. **文献名称**：*RAP80 Targets BRCA1 to Specific Ubiquitin Structures at DNA Damage Sites*
**作者**：Sobhian, B., et al. (2007)
**摘要**：该研究揭示RAP80通过其泛素相互作用基序(UIM)识别DNA损伤位点的K63泛素链，并引导BRCA1复合体定位到DNA双链断裂(DSB)处，对损伤修复至关重要。
2. **文献名称**：*The UIM Domain of RAP80 Mediates the Interaction with BRCA1 and Regulates G2/M Checkpoint*
**作者**：Yan, J., et al. (2007)
**摘要**：本文发现RAP80的UIM结构域直接结合BRCA1.并通过调控G2/M检验点维持基因组稳定性;研究还表明RAP80的磷酸化增强其与BRCA1的相互作用。
3. **文献名称**：*Structural and Functional Analysis of the RAP80-ABRAXAS Complex in DNA Damage Response*
**作者**：Wang, B., et al. (2009)
**摘要**：通过重组蛋白技术解析了RAP80与ABRAXAS的结构互作，证明该复合体是BRCA1招募到DSB位点的支架，且其功能依赖于RAP80的泛素识别能力。
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这些文献分别从功能机制(Sobhian 2007)、结构调控(Yan 2007)和复合体动态(Wang 2009)角度阐述了RAP80在DNA损伤应答中的核心作用。如需扩展，可补充《Genes & Development》中Kim等人(2007)关于ABRAXAS与RAP80协同工作的研究。
Recombinant human RAP80 (Receptor-Associated Protein 80) is a ubiquitin-interacting protein involved in DNA damage response and repair pathways. Discovered as a BRCA1-associated protein, RAP80 plays a critical role in recruiting the BRCA1-Abraxas-RAP80 complex to sites of DNA double-strand breaks (DSBs). It specifically recognizes K63-linked polyubiquitin chains through its tandem ubiquitin-interacting motifs (UIMs), a hallmark of DNA damage signaling. This targeting mechanism enables RAP80 to facilitate error-free DNA repair via homologous recombination while suppressing illegitimate recombination events. Structurally, RAP80 contains an N-terminal UIM domain, a middle ARM repeat region for protein interactions, and a C-terminal domain implicated in phosphorylation-dependent regulation. Its function extends beyond DSB repair, with emerging roles in cell cycle checkpoint control, genomic stability maintenance, and crosstalk with other DDR proteins like ATM and ATR. Dysregulation of RAP80 has been linked to cancer susceptibility, particularly in breast and ovarian cancers, where compromised DNA repair mechanisms contribute to tumorigenesis. Recombinant RAP80 proteins are widely used to study DNA repair mechanisms, screen therapeutic agents targeting DDR pathways, and investigate protein interaction networks in genome maintenance. Its conserved functional domains make it a valuable tool for understanding cancer biology and developing precision oncology strategies.
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