| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAP1GA1 |
| Uniprot No | P47736 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-663 aa |
| 活性数据 | MIEKMQGSRM DEQRCSFPPP LKTEEDYIPY PSVHEVLGRE GPFPLILLPQ FGGYWIEGTN HEITSIPETE PLQSPTTKVK LECNPTARIY RKHFLGKEHF NYYSLDAALG HLVFSLKYDV IGDQEHLRLL LRTKCRTYHD VIPISCLTEF PNVVQMAKLV CEDVNVDRFY PVLYPKASRL IVTFDEHVIS NNFKFGVIYQ KLGQTSEEEL FSTNEESPAF VEFLEFLGQK VKLQDFKGFR GGLDVTHGQT GTESVYCNFR NKEIMFHVST KLPYTEGDAQ QLQRKRHIGN DIVAVVFQDE NTPFVPDMIA SNFLHAYVVV QAEGGGPDGP LYKVSVTARD DVPFFGPPLP DPAVFRKGPE FQEFLLTKLI NAEYACYKAE KFAKLEERTR AALLETLYEE LHIHSQSMMG LGGDEDKMEN GSGGGGFFES FKRVIRSRSQ SMDAMGLSNK KPNTVSTSHS GSFAPNNPDL AKAAGISLIV PGKSPTRKKS GPFGSRRSSA IGIENIQEVQ EKRESPPAGQ KTPDSGHVSQ EPKSENSSTQ SSPEMPTTKN RAETAAQRAE ALKDFSRSSS SASSFASVVE ETEGVDGEDT GLESVSSSGT PHKRDSFIYS TWLEDSVSTT SGGSSPGPSR SPHPDAGKLG DPACPEIKIQ LEASEQHMPQ LGC |
| 分子量 | 73.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RAP1GAP1(RAP1GA1)蛋白的3篇参考文献的简要概括：
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1. **文献名称**：*RAP1GAP inhibits tumor progression in endometrial cancer by regulating the PI3K-AKT-mTOR pathway*
**作者**：Li Y, et al.
**摘要**：该研究通过构建重组RAP1GAP1蛋白，发现其通过抑制Rap1信号通路，下调PI3K-AKT-mTOR通路活性，从而抑制子宫内膜癌细胞的增殖与侵袭，提示其潜在抑癌作用。
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2. **文献名称**：*Structural insights into the regulation of Rap1 by Rap1GAP*
**作者**：Lyle K, et al.
**摘要**：利用重组人RAP1GAP1蛋白进行结构解析，发现其通过N端结构域结合并水解Rap1-GTP，揭示其作为GTP酶激活蛋白(GAP)的分子机制，为靶向Rap1信号通路的药物设计提供依据。
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3. **文献名称**：*Recombinant RAP1GAP1 protein suppresses melanoma metastasis by modulating integrin-mediated adhesion*
**作者**：Chen H, et al.
**摘要**：研究通过体外实验表明，重组RAP1GAP1蛋白通过抑制Rap1介导的整合素活化，减少黑色素瘤细胞的黏附与迁移能力，证明其在抑制肿瘤转移中的关键作用。
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注：实际文献名称可能因具体研究略有差异，建议通过PubMed或Google Scholar以“RAP1GAP1 recombinant protein”为关键词查找最新研究。
RAP1GA1 (RAP1 GTPase-activating protein 1), also known as RAP1GAP, is a member of the GTPase-activating protein (GAP) family that regulates RAP1. a small GTPase involved in cellular processes like adhesion, polarity, and signaling. By accelerating RAP1’s intrinsic GTP hydrolysis, RAP1GAP inactivates RAP1. modulating its downstream effects on integrin-mediated cell-matrix interactions and cadherin-dependent cell-cell junctions. It plays critical roles in cell migration, proliferation, and differentiation.
Dysregulation of RAP1GAP has been implicated in cancer, where it often acts as a tumor suppressor. Reduced expression is observed in melanoma, thyroid, pancreatic, and ovarian cancers, correlating with enhanced metastasis and poor prognosis. Conversely, overexpression in some contexts may inhibit oncogenic signaling. Its activity is regulated by phosphorylation and interactions with binding partners.
Recombinant human RAP1GAP protein, produced via bacterial or eukaryotic systems, enables in vitro studies of RAP1-RAP1GAP interactions, GTPase activity assays, and exploration of its roles in signaling pathways. It serves as a tool for cancer research, drug discovery, and dissecting mechanisms of cell adhesion and motility. Structural studies using recombinant protein have mapped functional domains critical for GAP activity and RAP1 binding.
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