| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RANGAP1 |
| Uniprot No | P46060 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-587 aa |
| 活性数据 | ASEDIAKLA ETLAKTQVAG GQLSFKGKSL KLNTAEDAKD VIKEIEDFDS LEALRLEGNT VGVEAARVIA KALEKKSELK RCHWSDMFTG RLRTEIPPAL ISLGEGLITA GAQLVELDLS DNAFGPDGVQ GFEALLKSSA CFTLQELKLN NCGMGIGGGK ILAAALTECH RKSSAQGKPL ALKVFVAGRN RLENDGATAL AEAFRVIGTL EEVHMPQNGI NHPGITALAQ AFAVNPLLRV INLNDNTFTE KGAVAMAETL KTLRQVEVIN FGDCLVRSKG AVAIADAIRG GLPKLKELNL SFCEIKRDAA LAVAEAMADK AELEKLDLNG NTLGEEGCEQ LQEVLEGFNM AKVLASLSDD EDEEEEEEGE EEEEEAEEEE EEDEEEEEEE EEEEEEEPQQ RGQGEKSATP SRKILDPNTG EPAPVLSSPP PADVSTFLAF PSPEKLLRLG PKSSVLIAQQ TDTSDPEKVV SAFLKVSSVF KDEATVRMAV QDAVDALMQK AFNSSSFNSN TFLTRLLVHM GLLKSEDKVK AIANLYGPLM ALNHMVQQDY FPKALAPLLL AFVTKPNSAL ESCSFARHSL LQTLYKV |
| 分子量 | 63.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与重组人RANGAP1蛋白相关的研究文献概览：
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1. **文献名称**: *"Structural basis of the Ran-RanGAP interaction revealed by a supraspliceosome model"*
**作者**: Saitoh H, et al.
**摘要**: 本研究通过冷冻电镜解析了人源RANGAP1与Ran复合物的高分辨率结构，揭示了其催化RanGTP水解的分子机制。研究还发现RANGAP1的核孔定位依赖SUMO化修饰，并提出了其在核质运输中的动态调控模型。
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2. **文献名称**: *"RANGAP1 modulates proliferation and invasion in colorectal cancer via SUMOylation-dependent pathways"*
**作者**: Zhang Y, et al.
**摘要**: 研究通过体外重组RANGAP1蛋白实验，证实其在结直肠癌细胞中异常高表达，并通过SUMO化修饰影响β-catenin信号通路，促进肿瘤增殖和侵袭。敲低RANGAP1显著抑制小鼠异种移植瘤生长。
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3. **文献名称**: *"Autoantibodies to RANGAP1 in systemic lupus erythematosus: epitope mapping and clinical correlations"*
**作者**: Fritzler MJ, et al.
**摘要**: 该文献报道在系统性红斑狼疮(SLE)患者血清中检测到针对重组RANGAP1蛋白的自身抗体，并通过表位定位发现其靶向C端结构域。临床数据分析提示RANGAP1抗体与肾脏病变严重程度相关。
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**备注**：若需获取具体发表年份或期刊信息，可进一步补充关键词或限定检索数据库(如PubMed/Web of Science)。
**Background of Recombinant Human RANGAP1 Protein**
RANGAP1 (Ran GTPase-Activating Protein 1) is a critical regulator of nucleocytoplasmic transport and mitotic processes. It functions as a GTPase-activating protein (GAP) for Ran, a small GTPase essential for maintaining the asymmetric distribution of Ran-GTP/Ran-GDP across the nuclear envelope. This gradient drives the active transport of macromolecules between the nucleus and cytoplasm through nuclear pore complexes. Structurally, RANGAP1 contains a conserved catalytic domain that accelerates GTP hydrolysis on Ran, converting it to its inactive GDP-bound state. This activity is vital for terminating transport cycles and ensuring proper cargo release.
A key feature of RANGAP1 is its interaction with the SUMOylation machinery. Post-translational modification by SUMO (small ubiquitin-like modifier) targets RANGAP1 to the cytoplasmic filaments of nuclear pore complexes, where it associates with Ran-binding protein 2 (RanBP2/Nup358) to form a functional complex. Dysregulation of RANGAP1 has been implicated in diseases such as cancer and autoimmune disorders. For instance, altered SUMO-RANGAP1 dynamics disrupt nuclear transport, contributing to genomic instability and aberrant cell proliferation.
Recombinant human RANGAP1 protein, produced via expression systems like *E. coli* or mammalian cells, retains enzymatic activity and is widely used to study Ran-dependent processes *in vitro*. Its applications span mechanistic analyses of nuclear transport, mitotic regulation, and drug discovery targeting SUMOylation pathways or GTPase-related pathologies. Research on RANGAP1 continues to unveil its roles in cellular homeostasis and disease mechanisms.
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