| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RANBP10 |
| Uniprot No | Q6VN20 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-620 aa |
| 活性数据 | AAATADPGA GNPQPGDSSG GGAGGGLPSP GEQELSRRLQ RLYPAVNQQE TPLPRSWSPK DKYNYIGLSQ GNLRVHYKGH GKNHKDAASV RATHPIPAAC GIYYFEVKIV SKGRDGYMGI GLSAQGVNMN RLPGWDKHSY GYHGDDGHSF CSSGTGQPYG PTFTTGDVIG CCVNLINGTC FYTKNGHSLG IAFTDLPANL YPTVGLQTPG EIVDANFGQQ PFLFDIEDYM REWRAKVQGT VHCFPISARL GEWQAVLQNM VSSYLVHHGY CATATAFARM TETPIQEEQA SIKNRQKIQK LVLEGRVGEA IETTQRFYPG LLEHNPNLLF MLKCRQFVEM VNGTDSEVRS LSSRSPKSQD SYPGSPSLSP RHGPSSSHMH NTGADSPSCS NGVASTKSKQ NHSKYPAPSS SSSSSSSSSS SSPSSVNYSE SNSTDSTKSQ HHSSTSNQET SDSEMEMEAE HYPNGVLGSM STRIVNGAYK HEDLQTDESS MDDRHPRRQL CGGNQAATER IILFGRELQA LSEQLGREYG KNLAHTEMLQ DAFSLLAYSD PWSCPVGQQL DPIQREPVCA ALNSAILESQ NLPKQPPLML ALGQASECLR LMARAGLGSC SFARVDDYLH |
| 分子量 | 67.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于人RANBP10蛋白的3篇参考文献的简要列举(注：文献信息为示例性概括，非真实存在)：
1. **文献名称**：*RANBP10 regulates cell migration and metastasis via interactions with microtubules in hepatocellular carcinoma*
**作者**：Xu, Z., et al.
**摘要**：该研究揭示RANBP10通过稳定微管网络促进肝癌细胞的迁移和侵袭，并证实其表达水平与患者预后不良相关。
2. **文献名称**：*RANBP10 is essential for spermatogenesis by mediating acrosome formation and nuclear shaping*
**作者**：Kawai, T., & Suzuki, A.
**摘要**：通过基因敲除模型发现，RANBP10在精子发生过程中参与顶体形成和细胞核形态重塑，缺失会导致雄性不育。
3. **文献名称**：*Structural insights into RANBP10’s role in nuclear transport and RNA splicing*
**作者**：Zhang, Y., et al.
**摘要**：通过晶体学分析发现RANBP10与RAN GTP酶及RNA剪接因子相互作用，提出其可能协调核质运输与RNA加工过程。
4. **文献名称**：*RANBP10 dysregulation links to myeloid leukemia via aberrant RAN signaling*
**作者**：Müller, S., et al.
**摘要**：研究发现RANBP10在急性髓系白血病中异常高表达，破坏RAN依赖的核转运通路，促进白血病细胞增殖。
**Background of Recombinant Human RANBP10 Protein**
RANBP10 (RAN Binding Protein 10) is a member of the RAN GTPase-interacting protein family, primarily involved in regulating nucleocytoplasmic transport, mitotic spindle assembly, and cell cycle progression. It binds to RAN, a small GTPase critical for maintaining the directional transport of macromolecules across the nuclear envelope by modulating its GTP/GDP state. Structurally, RANBP10 contains conserved domains, such as a C-terminal RAN-binding domain, enabling interactions with RAN-GTP, and additional motifs that facilitate associations with microtubules or other cytoskeletal components.
Studies suggest RANBP10 plays dual roles: in interphase, it may assist RAN in coordinating nuclear import/export, while during mitosis, it participates in spindle organization by linking RAN-GTP signaling to microtubule dynamics. Its interaction with motor proteins (e.g., kinesins) and Aurora kinases further highlights its regulatory importance in chromosomal segregation. Dysregulation of RANBP10 has been implicated in cancer, where overexpression correlates with enhanced cell proliferation, invasion, and chemoresistance, potentially due to altered spindle checkpoint control or aberrant transport pathways.
Recombinant human RANBP10 protein, produced via expression systems like *E. coli* or mammalian cells, serves as a tool to study these mechanisms *in vitro*, aiding drug discovery targeting RAN-dependent oncogenic processes. Research continues to explore its precise molecular interactions and therapeutic potential.
×
