| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RALGPS2 |
| Uniprot No | Q86X27 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-583 aa |
| 活性数据 | MDLMNGQASS VNIAATASEK SSSSESLSDK GSELKKSFDA VVFDVLKVTP EEYAGQITLM DVPVFKAIQP DELSSCGWNK KEKYSSAPNA VAFTRRFNHV SFWVVREILH AQTLKIRAEV LSHYIKTAKK LYELNNLHAL MAVVSGLQSA PIFRLTKTWA LLSRKDKTTF EKLEYVMSKE DNYKRLRDYI SSLKMTPCIP YLGIYLSDLT YIDSAYPSTG SILENEQRSN LMNNILRIIS DLQQSCEYDI PMLPHVQKYL NSVQYIEELQ KFVEDDNYKL SLKIEPGTST PRSAASREDL VGPEVGASPQ SGRKSVAAEG ALLPQTPPSP RNLIPHGHRK CHSLGYNFIH KMNTAEFKSA TFPNAGPRHL LDDSVMEPHA PSRGQAESST LSSGISIGSS DGSELSEETS WPAFERNRLY HSLGPVTRVA RNGYRSHMKA SSSAESEDLA VHLYPGAVTI QGVLRRKTLL KEGKKPTVAS WTKYWAALCG TQLFYYAAKS LKATERKHFK STSNKNVSVI GWMVMMADDP EHPDLFLLTD SEKGNSYKFQ AGNRMNAMLW FKHLSAACQS NKQQVPTNLM TFE |
| 分子量 | 65.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与重组人RALGPS2蛋白相关的文献摘要示例(基于公开研究趋势的模拟数据)：
1. **文献名称**：RALGPS2 regulates RalA activation and prostate cancer cell migration
**作者**：Smith et al. (2018)
**摘要**：研究揭示了RALGPS2通过激活RalA GTPase促进前列腺癌细胞迁移的机制，并通过重组蛋白实验证实其与细胞骨架重塑相关信号通路的相互作用。
2. **文献名称**：Structural insights into the PH domain of RALGPS2 and its lipid-binding activity
**作者**：Li et al. (2020)
**摘要**：利用重组RALGPS2蛋白解析其PH结构域的晶体结构，证明其在细胞膜定位中通过结合磷脂酰肌醇发挥功能，为靶向干预提供结构基础。
3. **文献名称**：RALGPS2 deficiency impairs endosomal trafficking and promotes tumorigenesis
**作者**：Chen & Wang (2021)
**摘要**：通过重组RALGPS2蛋白回补实验，证明其缺失导致内体运输异常，并通过调控RalB活性影响结直肠癌细胞增殖和转移。
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**注**：上述文献为模拟示例，实际研究中RALGPS2的直接功能报道较少，建议通过PubMed或Google Scholar以关键词“RALGPS2”或“RalGEF”筛选最新研究。如需真实文献，可进一步提供具体领域(如癌症、结构生物学等)以便精准检索。
**Recombinant Human RALGPS2 Protein: Background**
RALGPS2 (Ral Guanine Nucleotide Exchange Factor with PH Domain and SH3 Binding Motif 2) is a signaling regulator involved in cellular processes such as endocytosis, vesicle trafficking, cell migration, and proliferation. It acts as a guanine nucleotide exchange factor (GEF) for Ral GTPases, which are downstream effectors of the Ras signaling pathway. RALGPS2 contains a PH domain for membrane targeting and an SH3-binding motif for protein interactions, enabling its role in spatial regulation of Ral activation.
Studies highlight its dual role in cancer: it can act as a tumor suppressor or promoter depending on context. For example, RALGPS2 is downregulated in bladder and gastric cancers, correlating with poor prognosis, while its overexpression in certain models enhances metastatic potential. It also interacts with the Hippo-YAP pathway, influencing cell growth and organ size.
Recombinant RALGPS2 protein, produced via *in vitro* expression systems, is utilized to study its biochemical activity, structure-function relationships, and role in signaling networks. This tool aids in exploring its therapeutic potential, particularly in cancers with dysregulated RalGEF-Ral pathways. Research continues to unravel its context-dependent mechanisms and therapeutic implications.
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