| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAG2 |
| Uniprot No | P55895 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-527 aa |
| 活性数据 | MSLQMVTVSN NIALIQPGFS LMNFDGQVFF FGQKGWPKRS CPTGVFHLDV KHNHVKLKPT IFSKDSCYLP PLRYPATCTF KGSLESEKHQ YIIHGGKTPN NEVSDKIYVM SIVCKNNKKV TFRCTEKDLV GDVPEARYGH SINVVYSRGK SMGVLFGGRS YMPSTHRTTE KWNSVADCLP CVFLVDFEFG CATSYILPEL QDGLSFHVSI AKNDTIYILG GHSLANNIRP ANLYRIRVDL PLGSPAVNCT VLPGGISVSS AILTQTNNDE FVIVGGYQLE NQKRMICNII SLEDNKIEIR EMETPDWTPD IKHSKIWFGS NMGNGTVFLG IPGDNKQVVS EGFYFYMLKC AEDDTNEEQT TFTNSQTSTE DPGDSTPFED SEEFCFSAEA NSFDGDDEFD TYNEDDEEDE SETGYWITCC PTCDVDINTW VPFYSTELNK PAMIYCSHGD GHWVHAQCMD LAERTLIHLS AGSNKYYCNE HVEIARALHT PQRVLPLKKP PMKSLRKKGS GKILTPAKKS FLRRLFD |
| 分子量 | 59.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人RAG2蛋白的关键文献，内容涵盖其功能、结构及调控机制：
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1. **文献名称**：*V(D)J recombination: RAG proteins, repair factors, and regulation*
**作者**：David G. Schatz & Patrick C. Swanson
**摘要**：该综述系统性总结了RAG1/RAG2复合体在V(D)J重组中的作用，重点解析了RAG2通过其C端结构域与RAG1协同催化DNA断裂的分子机制，并讨论了其在适应性免疫中的核心地位(来源：*Annual Review of Biochemistry*, 2011)。
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2. **文献名称**：*Crystal structure of the RAG2 nonamer-binding domain reveals conservation of a V(D)J recombination module across evolution*
**作者**：Yanxiang Cui et al.
**摘要**：通过X射线晶体学技术揭示了RAG2蛋白的nonamer结合域(NBD)三维结构，发现其进化保守性及其在靶向特定DNA序列(如重组信号序列)中的关键功能，为理解RAG2如何精准介导V(D)J重组提供结构基础(来源：*Nature Structural & Molecular Biology*, 2005)。
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3. **文献名称**：*Cell cycle control of V(D)J recombination via RAG2 phosphorylation*
**作者**：Wei Li et al.
**摘要**：研究发现RAG2的磷酸化水平(尤其在Threonine 490位点)受细胞周期依赖性调控，确保其仅在G1期激活重组活性，从而防止基因组在DNA复制期受损，揭示了RAG2活性与细胞周期检查点的精密关联(来源：*Science*, 1996)。
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**附加文献(备选)**：
4. **文献名称**：*RAG2-deficient mice exhibit a novel form of immunodeficiency with severe B and T cell defects*
**作者**：S. A. Omori et al.
**摘要**：通过构建RAG2基因敲除小鼠模型，证实RAG2缺失导致淋巴细胞发育停滞于前体阶段，并伴随完全性V(D)J重组失败，验证了RAG2对免疫系统发育的必要性(来源：*Immunity*, 1998)。
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以上文献涵盖了RAG2的核心机制研究及临床应用探索，建议优先阅读Schatz团队及Cui等人的论文以深入理解其功能与结构。
**Background of Recombination-Activating Gene 2 (RAG2) Protein**
The Recombination-Activating Gene 2 (RAG2) protein, encoded by the *RAG2* gene, plays a critical role in the adaptive immune system by enabling V(D)J recombination, a process essential for generating diverse antigen receptors in lymphocytes. RAG2 works in concert with RAG1 to form a heterocomplex that catalyzes DNA cleavage at specific recombination signal sequences (RSSs) flanking antigen receptor gene segments. This activity is vital for assembling functional immunoglobulin and T-cell receptor genes during B- and T-cell development, ensuring immune diversity.
Structurally, RAG2 contains a non-catalytic N-terminal domain and a C-terminal plant homeodomain (PHD) finger that interacts with histone H3 trimethylated at lysine 4 (H3K4me3), linking recombination to epigenetic regulation. This interaction localizes the RAG complex to active chromatin regions, promoting precise DNA cleavage. While RAG1 mediates enzymatic activity, RAG2 stabilizes the complex and enhances substrate recognition.
Mutations in *RAG2* can cause severe combined immunodeficiency (SCID) or Omenn syndrome, disorders characterized by impaired lymphocyte maturation and immune dysfunction. Beyond its immunological role, RAG2 is studied for its involvement in genomic instability and lymphoid malignancies when dysregulated. Recombinant RAG2 proteins are widely used in research to dissect V(D)J mechanisms and model immune diseases, with emerging applications in gene therapy to correct RAG-related immunodeficiencies.
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