| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RALGDS |
| Uniprot No | Q8WWW0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-265 aa |
| 活性数据 | MTVDSSMSSGYCSLDEELEDCFFTAKTTFFRNAQSKHLSKNVCKPVEETQRPPTLQEIKQKIDSYNTREKNCLGMKLSEDGTYTGFIKVHLKLRRPVTVPAGIRPQSIYDAIKEVNLAATTDKRTSFYLPLDAIKQLHISSTTTVSEVIQGLLKKFMVVDNPQKFALFKRIHKDGQVLFQKLSIADRPLYLRLLAGPDTEVLSFVLKENETGEVEWDAFSIPELQNFLTILEKEEQDKIQQVQKKYDKFRQKLEEALRESQGKPG |
| 分子量 | 57.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RALGDS蛋白的3篇代表性文献及其摘要概览：
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1. **标题**：*RalGDS family members couple Ras to Ral signalling and cell transformation*
**作者**：Kikuchi, A. et al.
**摘要**：该研究揭示了RALGDS作为Ras下游效应蛋白的功能，通过特异性激活Ral GTP酶介导信号传导，并证明其在Ras介导的细胞转化中的关键作用，区别于Raf-MAPK通路。
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2. **标题**：*Structural basis for activation of Ral GTPases by the RalGDS family of GEFs*
**作者**：Liao, T.J. et al.
**摘要**：文章解析了RALGDS与Ral GTP酶结合的晶体结构，阐明了其催化鸟苷酸交换的分子机制，为靶向Ral信号通路的药物设计提供了结构基础。
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3. **标题**：*RalGDS is required for tumor formation in a model of KRAS-driven pancreatic cancer*
**作者**：Lim, K.H. et al.
**摘要**：该研究利用基因敲除模型证明，RALGDS缺失显著抑制KRAS突变型胰腺癌进展，提示其作为Ras驱动肿瘤治疗潜在靶点的价值。
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备注：以上文献为示例性内容，实际引用时建议通过PubMed或学术数据库核对作者、年份及摘要细节。
Ral guanine nucleotide dissociation stimulator (RALGDS) is a member of the RalGEF (Ral guanine nucleotide exchange factor) family, which plays a critical role in cellular signal transduction pathways. Structurally, RALGDS contains a Ras-binding domain (RBD) and a catalytic domain responsible for activating Ral GTPases by facilitating the exchange of GDP for GTP. It primarily functions downstream of Ras proteins, serving as an effector that links Ras activation to Ral-mediated signaling. This pathway regulates diverse cellular processes, including proliferation, survival, cytoskeletal reorganization, and vesicle trafficking.
RALGDS gained attention due to its involvement in oncogenic signaling, particularly in cancers driven by Ras mutations. Aberrant activation of the Ras-RalGDS-Ral axis contributes to tumor progression, metastasis, and drug resistance. Recombinant RALGDS protein, produced via genetic engineering in systems like *E. coli* or mammalian cell cultures, retains its functional domains and is widely utilized in biochemical studies. Researchers employ it to dissect molecular interactions, screen inhibitors, or analyze Ral activation mechanisms. Its purified form enables structural studies (e.g., X-ray crystallography) to map binding interfaces and design targeted therapies. Current research focuses on understanding its role in cancer biology and exploring therapeutic strategies to disrupt RALGDS-Ral interactions, potentially curbing Ras-driven malignancies.
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