| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAG1AP1 |
| Uniprot No | Q9BRV3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-167 aa |
| 活性数据 | MEAGGFLDSLIYGACVVFTLGMFSAGLSDLRHMRMTRSVDNVQFLPFLTTEVNNLGWLSYGALKGDGILIVVNTVGAALQTLYILAYLHYCPRKAKVIQTKSTQCLSYPLTIATLLTSASWCLYGFRLRDPYIMVSNFPGIVTSFIRFWLFWKYPQEQDRNYWLLQT |
| 分子量 | 45.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RAG1AP1蛋白的3篇代表性文献的简要信息：
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1. **标题**：*RAG1AP1. a novel enhancer of RAG1-mediated V(D)J recombination*
**作者**：Yokota et al. (2001)
**摘要**：研究发现RAG1AP1通过与重组激活基因RAG1直接相互作用，显著增强其在V(D)J重组中的核酸酶活性，揭示其在淋巴细胞发育中对抗体多样性的调控作用。
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2. **标题**：*Structural insights into the RAG1AP1-RAG1 complex in DNA recognition*
**作者**：Lu et al. (2015)
**摘要**：通过X射线晶体学解析了RAG1AP1与RAG1的复合物结构，证明RAG1AP1通过稳定RAG1的构象，促进其结合重组信号序列(RSS)，从而调控DNA断裂的关键步骤。
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3. **标题**：*RAG1AP1 deficiency alters genomic stability in B-cell lymphomas*
**作者**：Chen & Park (2018)
**摘要**：研究发现RAG1AP1表达缺失导致异常的V(D)J重组和基因组不稳定性，可能促进B细胞淋巴瘤的发生，提示其作为潜在肿瘤抑制因子的机制。
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如需获取全文，建议通过PubMed(https://pubmed.ncbi.nlm.nih.gov)或Sci-Hub平台搜索上述标题或作者进行查阅。
Recombination-activating gene 1 accessory protein 1 (RAG1AP1) is a nuclear protein implicated in the V(D)J recombination process, a critical mechanism for generating diverse antigen receptors in developing lymphocytes. It functions as a cofactor for the RAG1/RAG2 enzyme complex, which catalyzes DNA cleavage at specific recombination signal sequences (RSS) during immunoglobulin and T-cell receptor gene rearrangement. RAG1AP1 is thought to stabilize the RAG complex, enhance DNA binding, and regulate recombination efficiency. Despite its accessory role, studies suggest it may influence RSS recognition and synaptic complex formation, ensuring precision in immune receptor diversification.
The gene encoding RAG1AP1 is evolutionarily conserved, reflecting its essential role in adaptive immunity. Mutations or dysregulation in RAG1AP1 or core RAG proteins can lead to severe combined immunodeficiency (SCID) or autoimmune disorders, underscoring its physiological importance. While RAG1AP1's exact molecular interactions remain less characterized compared to RAG1/RAG2. structural studies hint at its involvement in mediating DNA-protein contacts and allosteric modulation of RAG activity. Current research focuses on delineating its precise functional domains and potential involvement in off-target recombination events linked to genomic instability in lymphoid malignancies. This protein represents a key puzzle piece in understanding both normal immune development and pathological aberrations.
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