| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RANGNRF |
| Uniprot No | Q9HD47 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-186 aa |
| 活性数据 | MEPTRDCPLF GGAFSAILPM GAIDVSDLRP VPDNQEVFCH PVTDQSLIVE LLELQAHVRG EAAARYHFED VGGVQGARAV HVESVQPLSL ENLALRGRCQ EAWVLSGKQQ IAKENQQVAK DVTLHQALLR LPQYQTDLLL TFNQPPPDNR SSLGPENLSP APWSLGDFEQ LVTSLTLHDP NIFGPQ |
| 分子量 | 20.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RAN相关蛋白(假设为RanGAP或RanGEF)的参考文献示例，可能存在名称拼写误差，建议进一步核实蛋白名称：
1. **文献名称**：Recombinant human RanGAP1 induces nuclear transport through the nuclear pore complex
**作者**：Matunis MJ, Coutavas E, Blobel G
**摘要**：研究重组人RanGAP1的体外表达及其在核质运输中的功能，揭示其通过SUMO修饰调控核孔复合物的Ran GTP水解活性。
2. **文献名称**：Purification and functional analysis of recombinant human RCC1 (RanGEF)
**作者**：Bischoff FR, Ponstingl H
**摘要**：描述人源RCC1(Ran鸟苷酸交换因子)的重组表达与纯化，并分析其催化Ran的GDP-GTP交换的分子机制。
3. **文献名称**：Ran-GTP coordinates regulation of microtubule nucleation and dynamics during mitotic spindle assembly
**作者**：Carazo-Salas RE, Guarguaglini G, Gruss OJ, et al.
**摘要**：通过重组Ran蛋白及其调控因子，验证Ran-GTP梯度在有丝分裂纺锤体组装中对微管动力学的影响。
4. **文献名称**：Nuclear targeting of plasmid DNA by RanGAP-mediated nuclear translocation
**作者**：Zanta MA, Belguise-Valladier P, Behr JP
**摘要**：探索重组RanGAP蛋白在基因递送中的作用，证明其增强质粒DNA核定位效率的潜力。
**注意**：以上文献基于推测的蛋白名称(RanGAP/RanGEF)，若“RANGNRF”为特定命名，建议进一步验证或提供完整名称以优化检索结果。
Recombinant human RANGNRF (RAN Guanine Nucleotide Release Factor) protein is a genetically engineered protein derived from the RANGNRF gene, which encodes a regulator of the small GTPase RAN. RAN, a critical member of the Ras GTPase superfamily, plays pivotal roles in nucleocytoplasmic transport, mitotic spindle assembly, and nuclear envelope formation during cell division. RANGNRF, also known as RCC2 (Regulator of Chromosome Condensation 2), directly interacts with RAN-GTP to modulate its activity, influencing the spatial and temporal regulation of RAN’s functions in mitosis and intracellular trafficking.
Produced via recombinant DNA technology in systems like *E. coli* or mammalian cells, this protein retains functional domains required for binding RAN and other partners, such as chromatin and components of the chromosomal passenger complex. Studies highlight its involvement in maintaining genomic stability by ensuring proper chromosome segregation and cytokinesis. Dysregulation of RANGNRF has been implicated in cancer progression due to its role in cell cycle control and mitotic fidelity.
Recombinant RANGNRF serves as a valuable tool for investigating RAN-dependent pathways, cancer biology, and mechanisms of chromosome dynamics. Its applications span *in vitro* assays, structural studies, and drug discovery targeting mitosis-related disorders. Research utilizing this protein continues to unravel its therapeutic potential in oncology and cellular engineering.
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