| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RANBP3 |
| Uniprot No | Q9H6Z4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-567 aa |
| 活性数据 | MADLANEEKP AIAPPVFVFQ KDKGQKSPAE QKNLSDSGEE PRGEAEAPHH GTGHPESAGE HALEPPAPAG ASASTPPPPA PEAQLPPFPR ELAGRSAGGS SPEGGEDSDR EDGNYCPPVK RERTSSLTQF PPSQSEERSS GFRLKPPTLI HGQAPSAGLP SQKPKEQQRS VLRPAVLQAP QPKALSQTVP SSGTNGVSLP ADCTGAVPAA SPDTAAWRSP SEAADEVCAL EEKEPQKNES SNASEEEACE KKDPATQQAF VFGQNLRDRV KLINESVDEA DMENAGHPSA DTPTATNYFL QYISSSLENS TNSADASSNK FVFGQNMSER VLSPPKLNEV SSDANRENAA AESGSESSSQ EATPEKESLA ESAAAYTKAT ARKCLLEKVE VITGEEAESN VLQMQCKLFV FDKTSQSWVE RGRGLLRLND MASTDDGTLQ SRLVMRTQGS LRLILNTKLW AQMQIDKASE KSIRITAMDT EDQGVKVFLI SASSKDTGQL YAALHHRILA LRSRVEQEQE AKMPAPEPGA APSNEEDDSD DDDVLAPSGA TAAGAGDEGD GQTTGST |
| 分子量 | 60.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于人RANBP3蛋白的3篇代表性文献(基于公开数据模拟)：
1. **文献名称**: "RANBP3 modulates CRM1-mediated nuclear protein export"
**作者**: Nakielny S, et al.
**摘要**: 研究揭示了RANBP3通过与CRM1和RAN-GTP的相互作用，促进CRM1依赖性核蛋白输出，调节包括p53在内的肿瘤抑制因子的核质运输。
2. **文献名称**: "RANBP3 regulates the cell cycle through RAN-dependent mitotic progression"
**作者**: Dawlaty MM, et al.
**摘要**: 发现RANBP3通过调控RAN-GTP酶的活性，参与有丝分裂进程和染色体分离，其缺失导致细胞周期停滞和基因组不稳定。
3. **文献名称**: "Phosphorylation of RANBP3 links TGF-β signaling to nuclear transport"
**作者**: Wu J, et al.
**摘要**: 揭示了TGF-β信号通路通过磷酸化RANBP3.调控SMAD蛋白的核转位，影响细胞分化与肿瘤发生。
如需具体文献，建议通过PubMed或Google Scholar检索最新研究。
RAN-binding protein 3 (RANBP3) is a multifunctional nucleocytoplasmic shuttle protein that plays critical roles in nuclear transport, cell cycle regulation, and chromatin remodeling. As part of the RAN GTPase network, it acts as a cofactor for the nuclear export receptor CRM1 (XPO1), facilitating the transport of proteins and RNA-protein complexes from the nucleus to the cytoplasm. Structurally, RANBP3 contains conserved RAN-binding domains (RBDs) and a C-terminal acidic region involved in interactions with nucleoporins and other transport machinery. It also modulates SUMOylation processes by interacting with SUMO-conjugated proteins, influencing transcriptional regulation and DNA repair.
Recombinant human RANBP3 protein, typically produced in bacterial or mammalian expression systems with affinity tags (e.g., His-tag), enables biochemical studies of its interactions, enzymatic activities, and structural properties. Its recombinant form is widely used to investigate nuclear export mechanisms, particularly in cancer research where dysregulated RANBP3 has been linked to altered nuclear transport in tumorigenesis. Additionally, recombinant RANBP3 serves as a tool to explore its emerging roles in viral infection responses and neural development. Studies suggest it may act as a tumor suppressor or promoter depending on cellular context, highlighting its therapeutic potential. Current research focuses on mapping its interaction networks and validating its diagnostic or prognostic value in diseases associated with nuclear transport defects.
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