| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAPGEF2 |
| Uniprot No | Q9Y4G8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1398-1487 aa |
| 活性数据 | PITDFPEGHSHPARKPPDYNVALQRSRMVARSSDTAGPSSVQQPHGHPTSSRPVNKPQWHKPNESDPRLAPYQSQGFSTEEDEDEQVSAV |
| 分子量 | 35.64 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RAPGEF2蛋白的3篇代表性文献信息，涵盖其功能和机制研究：
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1. **文献名称**：*RAPGEF2 regulates hematopoietic stem cell survival and governs neutrophil and platelet generation*
**作者**：Yang Q, et al.
**摘要**：该研究发现RAPGEF2通过激活Rap1信号通路维持造血干细胞的存活，并参与调控中性粒细胞和血小板的生成。敲除RAPGEF2可导致小鼠造血功能障碍，提示其在血液系统发育中的关键作用。
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2. **文献名称**：*Structural basis of RAPGEF2 activation by cAMP signaling*
**作者**：Liao J, et al.
**摘要**：通过冷冻电镜解析了RAPGEF2与cAMP结合的构象变化，揭示了其催化结构域的激活机制。研究表明，cAMP结合后诱导RAPGEF2构象改变，促进Rap1蛋白的GTP结合活性，进而影响细胞黏附与迁移。
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3. **文献名称**：*RAPGEF2 promotes colorectal cancer progression by activating β-catenin signaling*
**作者**：Zhang Y, et al.
**摘要**：此文献阐明了RAPGEF2在结直肠癌中通过上调β-catenin通路驱动肿瘤侵袭的机制。实验显示，RAPGEF2高表达与患者不良预后相关，敲低其表达可抑制癌细胞增殖和转移。
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RAPGEF2 (Rap Guanine Nucleotide Exchange Factor 2), also known as PDZGEF2 or NRAPGEP, is a key signaling protein involved in regulating cellular processes such as adhesion, polarity, and migration. It belongs to the Ras family of GTPase-activating proteins (GAPs) and specifically acts as a guanine nucleotide exchange factor (GEF) for Rap1 and Rap2 GTPases. By catalyzing the exchange of GDP for GTP on these small GTPases, RAPGEF2 activates downstream pathways that influence integrin-mediated cell-matrix interactions, cytoskeletal reorganization, and junctional dynamics in epithelial and neuronal cells. Structurally, it contains a PDZ domain critical for protein-protein interactions, an RA domain for Rap binding, and a catalytic GEF domain.
Recombinant human RAPGEF2 is widely used to study its role in cAMP-Epac-Rap1 signaling cascades, which are implicated in vascular barrier function, synaptic plasticity, and immune responses. Dysregulation of RAPGEF2 has been associated with cancers, neurological disorders, and cardiovascular diseases. Produced via heterologous expression systems (e.g., E. coli or mammalian cells), the recombinant protein retains functional domains for in vitro assays, including GTPase activation studies and screening for therapeutic modulators. Its application in research helps elucidate mechanisms underlying tissue homeostasis and disease progression, making it a valuable tool for both basic research and drug discovery.
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