| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RASEF |
| Uniprot No | Q8IZ41 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-740 aa |
| 活性数据 | MEADGDGEEL ARLRSVFAAC DANRSGRLER EEFRALCTEL RVRPADAEAV FQRLDADRDG AITFQEFARG FLGSLRGGRR RDWGPLDPAP AVSEAGPETH DSEEDEGDED AAAALATSCG PASPGRAWQD FQARLGDEAK FIPREEQVST LYQNINLVEP RLIQPYEHVI KNFIREIRLQ STEMENLAIA VKRAQDKAAM QLSELEEEMD QRIQAAEHKT RKDEKRKAEE ALSDLRRQYE TEVGDLQVTI KKLRKLEEQS KRVSQKEDVA ALKKQIYDLS MENQKVKKDL LEAQTNIAFL QSELDALKSD YADQSLNTER DLEIIRAYTE DRNSLERQIE ILQTANRKLH DSNDGLRSAL ENSYSKFNRS LHINNISPGN TISRSSPKFI GHSPQPLGYD RSSRSSYVDE DCDSLALCDP LQRTNCEVDS LPESCFDSGL STLRDPNEYD SEVEYKHQRG FQRSHGVQES FGGDASDTDV PDIRDEETFG LEDVASVLDW KPQGSVSEGS IVSSSRKPIS ALSPQTDLVD DNAKSFSSQK AYKIVLAGDA AVGKSSFLMR LCKNEFRENI SATLGVDFQM KTLIVDGERT VLQLWDTAGQ ERFRSIAKSY FRKADGVLLL YDVTCEKSFL NIREWVDMIE DAAHETVPIM LVGNKADIRD TAATEGQKCV PGHFGEKLAM TYGALFCETS AKDGSNIVEA VLHLAREVKK RTDKDDSRSI TNLTGTNSKK SPQMKNCCNG |
| 分子量 | 82.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RASEF蛋白的3条文献摘要信息(文献为虚构示例，供参考)：
1. **《RASEF蛋白在黑色素瘤中的异常表达及功能研究》**
- 作者：Müller A. et al.
- 摘要：研究发现RASEF作为RAS家族成员，在黑色素瘤中表达显著下调，重组RASEF蛋白可通过抑制MAPK信号通路抑制肿瘤迁移和增殖，提示其潜在抑癌作用。
2. **《重组RASEF蛋白与cAMP信号通路的相互作用》**
- 作者：Zhang L. et al.
- 摘要：通过体外实验证实重组RASEF蛋白能够结合EF-hand结构域调控细胞内cAMP水平，影响纤毛形成及细胞极性，为研究纤毛相关疾病提供新靶点。
3. **《RASEF在小鼠前列腺癌模型中的血管生成调节功能》**
- 作者：Johnson R. et al.
- 摘要：重组RASEF蛋白被证实通过调控VEGF通路抑制肿瘤血管生成，可能成为抗血管生成治疗的潜在分子。
注：以上文献为模拟内容，实际研究需查询真实数据库(如PubMed)获取。
**Background of RASEF Protein**
RASEF, or **RAS and EF-Hand Domain Containing Protein**, is a member of the RAS superfamily of small GTPases, which are critical regulators of intracellular signaling pathways. Unlike classical RAS proteins, RASEF uniquely combines a RAS-associated domain with a C-terminal EF-hand calcium-binding motif, suggesting its functional interplay between calcium signaling and GTPase activity. It was first identified in retinal photoreceptors but is expressed in various tissues, including the kidney, lung, and adipose tissue.
RASEF is implicated in diverse cellular processes such as cell proliferation, differentiation, vesicle trafficking, and apoptosis. Its dual-domain structure allows it to act as a molecular switch, coupling calcium signals to GTPase-dependent pathways like MAPK or PI3K/AKT, potentially modulating metabolic or stress responses. In cancer, RASEF exhibits context-dependent roles, acting as either a tumor suppressor or promoter. For example, it is downregulated in melanoma and renal cell carcinoma, correlating with poor prognosis, yet overexpressed in some lung and prostate cancers.
Studies also highlight its involvement in metabolic disorders. RASEF regulates adipocyte differentiation and mitochondrial function in brown fat, linking it to obesity and diabetes. Despite these findings, its precise mechanisms remain under investigation, emphasizing its potential as a therapeutic target or biomarker in cancer and metabolic diseases.
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