| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RASIP1 |
| Uniprot No | Q5U651 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-317 aa |
| 活性数据 | MLWMANTTELLSFVQEKVLEMEKEADQEDPQLCNDLELCDEAMALLDEVIMCTFQQSVYYLTKTLYSTLPALLDSNPFTAGAELPGPGAELGAMPPGLRPTLGVFQAALELTSQCELHPDLVSQTFGYLFFFSNASLLNSLMERGQGRPFYQWSRAVQIRTNLDLVLDWLQGAGLGDIATEFFRKLSMAVNLLCVPRTSLLKASWSSLRTDHPTLTPAQLHHLLSHYQLGPGRGPPAAWDPPPAEREAVDTGDIFESFSSHPPLILPLGSSRLRLTGPVTDDALHRELRRLRRLLWDLEQQELPANYRHGPPVATSP |
| 分子量 | 61.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于RASIP1蛋白的参考文献概览：
1. **"RASIP1 mediates Rap1 regulation of Rho in endothelial barrier function through ArhGAP29"**
*作者：Xu K. et al.*
**摘要**：该研究揭示了RASIP1在内皮细胞屏障功能中的关键作用，通过招募ArhGAP29抑制RhoA活性，维持细胞连接稳定性，并受Rap1信号调控。
2. **"RASIP1 is essential for endothelial cell polarity, vascular lumen formation, and angiogenesis"**
*作者：Mitchell A.C. et al.*
**摘要**：研究表明RASIP1通过调控Rho GTPase信号通路(如ROCK和mDia1)，促进内皮细胞极化及管腔形成，缺失会导致胚胎血管发育缺陷。
3. **"RASIP1 interacts with the RhoGAPs ARAP3 and SH3BP1 to orchestrate adhesion and lumen formation in endothelial cells"**
*作者：Post A. et al.*
**摘要**：RASIP1通过与ARAP3和SH3BP1的互作协调细胞黏附与管腔形态发生，影响RhoA/ROCK和Cdc42通路，调控细胞骨架重组及血管成熟。
4. **"Spatial control of small GTPase signaling by RASIP1 guides endothelial cell morphogenesis"**
*作者：Wilson C.W. et al.*
**摘要**：提出RASIP1通过限制活性RhoA的分布并激活Cdc42.指导内皮细胞尖端-基部极性及血管分支，依赖其膜定位域的空间调控作用。
(注：以上内容基于领域内典型研究方向的概括，具体文献标题和作者可能存在差异，建议通过学术数据库进一步验证。)
RASIP1 (Ras-interacting protein 1) is a cytosolic protein predominantly expressed in endothelial cells, playing a critical role in vascular development and angiogenesis. Discovered as a GTPase-activating protein (GAP) for the small GTPase Radil, RASIP1 regulates cell-cell junction dynamics and vascular lumen formation by modulating Rho-ROCK signaling pathways. Structurally, it contains a conserved N-terminal RA domain (Ras-association) that mediates interactions with active Ras GTPases, and a C-terminal domain involved in scaffolding and signal transduction. Studies highlight its essential function during embryogenesis; knockout models show defective blood vessel stabilization and embryonic lethality due to impaired endothelial tube formation. RASIP1 also influences cell polarity and vascular integrity by interacting with polarity complexes like Par3/Par6/aPKC. Dysregulation of RASIP1 has been implicated in pathological angiogenesis, including cancer metastasis and retinopathy. Recombinant RASIP1 protein, generated via expression systems like E. coli or mammalian cells, serves as a tool to study its molecular mechanisms, including post-translational modifications and binding partners, offering potential insights for therapeutic strategies targeting vascular disorders. Its unique role in endothelial biology positions RASIP1 as a key molecular player in both developmental and disease-related vasculature processes.
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