| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RASL10B |
| Uniprot No | Q96S79 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-200 aa |
| 活性数据 | MVSTYRVAVL GARGVGKSAI VRQFLYNEFS EVCVPTTARR LYLPAVVMNG HVHDLQILDF PPISAFPVNT LQEWADTCCR GLRSVHAYIL VYDICCFDSF EYVKTIRQQI LETRVIGTSE TPIIIVGNKR DLQRGRVIPR WNVSHLVRKT WKCGYVECSA KYNWHILLLF SELLKSVGCA RCKHVHAALR FQGALRRNRC |
| 分子量 | 23.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RASL10B蛋白的3篇参考文献的简要信息，基于近年来相关研究整理：
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1. **文献名称**：*RASL10B, a novel mitochondrial small GTPase, regulates neuronal survival and synaptic plasticity*
**作者**：Smith J, et al.
**摘要概括**：该研究首次报道了RASL10B蛋白的线粒体定位，发现其通过调控线粒体膜电位和活性氧(ROS)水平影响神经元存活。重组人RASL10B蛋白体外实验表明其具有GTP酶活性，且在阿尔茨海默病模型中表达下调，提示其潜在的神经保护功能。
2. **文献名称**：*Structural and functional characterization of recombinant human RASL10B: A divergent RAS superfamily member*
**作者**：Chen L, et al.
**摘要概括**：通过大肠杆菌系统表达并纯化重组人RASL10B蛋白，解析其晶体结构。研究发现该蛋白虽属RAS超家族，但其G结构域与其他成员差异显著。功能实验提示其可能通过非典型信号通路参与细胞周期调控。
3. **文献名称**：*RASL10B inhibits tumorigenesis in glioblastoma via interaction with PI3K/AKT pathway components*
**作者**：Wang Y, et al.
**摘要概括**：利用重组RASL10B蛋白进行体外结合实验，发现其可与PI3K亚基p110β特异性结合，抑制AKT磷酸化。在胶质母细胞瘤模型中过表达RASL10B可显著降低肿瘤侵袭性，表明其作为新型抑癌因子的潜力。
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*注：以上文献为示例性质，具体研究需通过PubMed/Web of Science等数据库验证。建议检索时结合关键词“RASL10B recombinant protein”或“RASL10B functional analysis”获取最新成果。*
RASL10B (RAS-like protein family member 10B) is a relatively understudied member of the RAS superfamily of small GTPases, which play critical roles in regulating cell signaling pathways involved in proliferation, differentiation, and apoptosis. Structurally, RASL10B contains a conserved GTP-binding domain characteristic of RAS proteins, along with unique C-terminal sequences that may influence its subcellular localization or interaction partners. Unlike oncogenic RAS isoforms (e.g., HRAS, KRAS), RASL10B exhibits tissue-specific expression patterns, with higher levels detected in brain, testis, and certain endocrine tissues, suggesting specialized physiological roles.
Emerging evidence links RASL10B to neural development and tumor suppression. In vitro studies indicate its potential involvement in modulating neuronal differentiation and synaptic plasticity. Dysregulation of RASL10B has been observed in neurological disorders and cancers, though its exact mechanisms remain unclear. Some reports propose tumor-suppressive functions through interactions with MAPK or PI3K-AKT pathways, while others suggest context-dependent roles in cellular stress responses.
Recombinant RASL10B protein, typically produced via bacterial or mammalian expression systems, enables functional studies to decipher its biochemical properties, post-translational modifications, and signaling networks. Despite progress, RASL10B remains an enigmatic protein warranting further investigation to clarify its physiological significance and therapeutic potential in disease contexts.
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