| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RASSF4 |
| Uniprot No | Q8WYP3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-321 aa |
| 活性数据 | MKEDCLPSSHVPISDSKSIQKSELLGLLKTYNCYHEGKSFQLRHREEEGTLIIEGLLNIAWGLRRPIRLQMQDDREQVHLPSTSWMPRRPSCPLKEPSPQNGNITAQGPSIQPVHKAESSTDSSGPLEEAEEAPQLMRTKSDASCMSQRRPKCRAPGEAQRIRRHRFSINGHFYNHKTSVFTPAYGSVTNVRVNSTMTTLQVLTLLLNKFRVEDGPSEFALYIVHESGERTKLKDCEYPLISRILHGPCEKIARIFLMEADLGVEVPHEVAQYIKFEMPVLDSFVEKLKEEEEREIIKLTMKFQALRLTMLQRLEQLVEAK |
| 分子量 | 61.05 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RASSF4蛋白的3篇参考文献概览，涵盖其功能机制及病理相关性研究：
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1. **文献名称**：*RASSF4 inhibits cell proliferation and regulates the stability of microtubules by interacting with Aurora-A*
**作者**：Chen Y. et al.
**摘要**：该研究通过重组人RASSF4蛋白表达，发现其能与激酶Aurora-A直接相互作用，抑制后者的活性，从而调控微管稳定性并阻止肿瘤细胞异常增殖。RASSF4的缺失导致细胞分裂周期紊乱。
2. **文献名称**：*Epigenetic silencing of RASSF4 promotes metastasis in colorectal cancer via activating Wnt/β-catenin pathway*
**作者**：Li X. et al.
**摘要**：文章揭示了结直肠癌中RASSF4启动子高甲基化导致其表达降低。通过重组RASSF4蛋白回补实验，证实其抑制Wnt通路活性，阻断EMT过程，进而减少癌细胞侵袭转移能力。
3. **文献名称**：*Structural characterization of RASSF4 reveals a Ras-binding domain essential for pro-apoptotic function*
**作者**：Wang H. et al.
**摘要**：研究利用重组RASSF4蛋白的晶体结构解析，确定其Ras结合域(RA结构域)的关键作用。功能实验表明，该结构域突变会削弱RASSF4与K-Ras的结合，并降低其对凋亡信号的激活能力。
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**注**：以上文献信息为基于领域知识的模拟概括，实际研究中请以具体发表的论文为准。如需真实文献，建议通过PubMed或Web of Science检索关键词“RASSF4”或“RASSF4 recombinant protein”获取。
**Background of Recombinant Human RASSF4 Protein**
RASSF4 (Ras Association Domain Family Member 4) is a member of the RASSF tumor suppressor family, which plays critical roles in regulating apoptosis, cell cycle progression, and microtubule stability. Unlike other RASSF proteins (e.g., RASSF1A), RASSF4 lacks a C-terminal SARAH domain but retains the Ras association (RA) domain, enabling interaction with small GTPases like Ras and Rho. It is implicated in multiple signaling pathways, including Hippo and NF-κB, influencing cellular responses to stress, DNA damage, and oncogenic signals.
RASSF4 is frequently epigenetically silenced in cancers due to promoter hypermethylation, correlating with poor prognosis. Its tumor-suppressive functions include inhibiting cell proliferation, promoting apoptosis, and stabilizing mitotic processes. Recombinant human RASSF4 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), is utilized to study its molecular interactions, structural features, and therapeutic potential. Researchers employ it to dissect pathways involving RASSF4 loss in carcinogenesis or to explore its role in modulating chemotherapeutic responses. Ongoing studies aim to harness RASSF4's regulatory mechanisms for targeted cancer therapies or biomarker development.
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