| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RBM19 |
| Uniprot No | Q9Y4C8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-960 aa |
| 活性数据 | MSRLIVKNLPNGMKEERFRQLFAAFGTLTDCSLKFTKDGKFRKFGFIGFKSEEEAQKAQKHFNKSFIDTSRITVEFCKSFGDPAKPRAWSKHAQKPSQPKQPPKDSTTPEIKKDEKKKKVAGQLEKLKEDTEFQEFLSVHQRRAQAATWANDGLDAEPSKGKSKPASDYLNFDSDSGQESEEEGAGEDLEEEASLEPKAAVQKELSDMDYLKSKMVKAGSSSSSEEEESEDEAVHCDEGSEAEEEDSSATPVLQERDSKGAGQEQGMPAGKKRPPEARAETEKPANQKEPTTCHTVKLRGAPFNVTEKNVMEFLAPLKPVAIRIVRNAHGNKTGYIFVDFSNEEEVKQALKCNREYMGGRYIEVFREKNVPTTKGAPKNTTKSWQGRILGENEEEEDLAESGRLFVRNLPYTSTEEDLEKLFSKYGPLSELHYPIDSLTKKPKGFAFITFMFPEHAVKAYSEVDGQVFQGRMLHVLPSTIKKEASEDASALGSSSYKKKKEAQDKANSASSHNWNTLFMGPNAVADAIAQKYNATKSQVFDHETKGSVAVRVALGETQLVQEVRRFLIDNGVSLDSFSQAAAERSKTVILVKNLPAGTLAAELQETFGHFGSLGRVLLPEGGITAIVEFLEPLEARKAFRHLAYSKFHHVPLYLEWAPVGVFSSTAPQKKKLQDTPSEPMEKDPAEPETVPDGETPEDENPTEEGADNSSAKMEEEEEEEEEEEESLPGCTLFIKNLNFDTTEEKLKEVFSKVGTVKSCSISKKKNKAGVLLSMGFGFVEYRKPEQAQKALKQLQGHVVDGHKLEVRISERATKPAVTLARKKQVPRKQTTSKILVRNIPFQAHSREIRELFSTFGELKTVRLPKKMTGTGTHRGFGFVDFLTKQDAKRAFNALCHSTHLYGRRLVLEWADSEVTLQALRQKTAAHFHEPPKKKRSVVLDEILEQLEGSDSDSEEQTLQL |
| 分子量 | 133.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RBM19蛋白的3篇参考文献示例，基于相关领域的研究方向归纳。由于文献的实时性和具体内容需通过学术数据库核实，建议进一步检索PubMed、Web of Science等平台获取准确信息。
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1. **文献名称**：*RBM19 modulates ribosome RNA processing and cellular proliferation through RNA-binding activity*
**作者**：Zhang Y, et al.
**摘要**：研究揭示了RBM19通过其RNA结合结构域参与核糖体RNA(rRNA)的加工过程，调控核仁内rRNA前体的剪切成熟，并影响细胞周期进程。基因敲除实验表明，RBM19缺失会导致细胞增殖能力显著下降。
2. **文献名称**：*Structural and functional characterization of recombinant human RBM19 in spliceosome assembly*
**作者**：Lee S, et al.
**摘要**：该研究通过重组表达纯化了人源RBM19蛋白，结合冷冻电镜技术解析其与剪接体复合物的相互作用，发现RBM19通过特定结构域促进mRNA剪接的准确性，为剪接调控机制提供新见解。
3. **文献名称**：*RBM19 mutations disrupt neurodevelopment via aberrant RNA splicing in zebrafish models*
**作者**：Wang X, et al.
**摘要**：利用斑马鱼模型，研究发现RBM19突变会干扰胚胎神经发育，导致RNA剪接异常。实验表明重组RBM19蛋白能部分恢复剪接缺陷，提示其潜在治疗应用价值。
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**备注**：上述文献为示例性质，具体内容需以实际发表论文为准。建议结合关键词“RBM19”、“RNA-binding protein”、“recombinant protein”及“splicing/ribosome”等在学术数据库中检索最新成果。
**Background of Recombinant Human RBM19 Protein**
Recombinant human RBM19 (RNA Binding Motif Protein 19) is a genetically engineered protein derived from the human *RBM19* gene, which encodes an RNA-binding protein critical for ribosomal biogenesis and RNA processing. RBM19 contains multiple RNA recognition motifs (RRMs) and is predominantly localized in the nucleolus, where it facilitates rRNA maturation, spliceosome assembly, and ribosome subunit formation. Studies suggest its involvement in cell cycle regulation, p53 signaling, and stress response pathways.
Dysregulation of RBM19 has been linked to diseases, including cancer, where mutations or altered expression may impair ribosome synthesis, leading to genomic instability. Recombinant RBM19 is widely used to study its molecular interactions, RNA-binding specificity, and functional roles in vitro. Its production via bacterial or mammalian expression systems enables structural analysis, biochemical assays, and exploration of its potential as a therapeutic target. Current research focuses on elucidating its role in tumorigenesis, ribosomopathies, and developmental disorders, highlighting its significance in cellular homeostasis and disease mechanisms.
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