| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RBM46 |
| Uniprot No | Q8TBY0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-533 aa |
| 活性数据 | MNEENIDGTNGCSKVRTGIQNEAALLALMEKTGYNMVQENGQRKFGGPPPGWEGPPPPRGCEVFVGKIPRDMYEDELVPVFERAGKIYEFRLMMEFSGENRGYAFVMYTTKEEAQLAIRILNNYEIRPGKFIGVCVSLDNCRLFIGAIPKEKKKEEILDEMKKVTEGVVDVIVYPSATDKTKNRGFAFVEYESHRAAAMARRKLIPGTFQLWGHTIQVDWADPEKEVDEETMQRVKVLYVRNLMISTTEETIKAEFNKFKPGAVERVKKLRDYAFVHFFNREDAVAAMSVMNGKCIDGASIEVTLAKPVNKENTWRQHLNGQISPNSENLIVFANKEESHPKTLGKLPTLPARLNGQHSPSPPEVERCTYPFYPGTKLTPISMYSLKSNHFNSAVMHLDYYCNKNNWAPPEYYLYSTTSQDGKVLLVYKIVIPAIANGSQSYFMPDKLCTTLEDAKELAAQFTLLHLDYNFHRSSINSLSPVSATLSSGTPSVLPYTSRPYSYPGYPLSPTISLANGSHVGQRLCISNQASFF |
| 分子量 | 66.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RBM46蛋白的3-4篇文献示例及其核心摘要内容：
1. **文献名称**：*RBM46 is essential for spermatogenesis and binds in vivo to specific transcripts in male germ cells*
**作者**：Chen et al. (2020)
**摘要**：通过重组RBM46蛋白及小鼠模型，揭示其在精子发生中的关键作用，通过结合生殖细胞mRNA调控减数分裂与染色体重塑。
2. **文献名称**：*Expression and purification of recombinant human RBM46 protein for structural studies*
**作者**：Smith et al. (2019)
**摘要**：报道了人源RBM46蛋白在大肠杆菌中的重组表达与纯化方法，解析其RNA结合结构域的晶体结构，阐述RNA互作机制。
3. **文献名称**：*Functional analysis of RBM46 in mammalian germ cell development using a knockout model*
**作者**：Wang et al. (2021)
**摘要**：基于RBM46敲除小鼠，证明其缺失导致生殖细胞发育缺陷，重组蛋白可部分恢复基因表达异常，支持其在转录后调控中的功能。
4. **文献名称**：*RNA-binding motif protein 46 regulates alternative splicing during mouse oocyte maturation*
**作者**：Li et al. (2018)
**摘要**：发现重组RBM46蛋白通过调控pre-mRNA剪接，影响卵母细胞减数分裂相关基因表达，依赖其RNA结合基序招募剪接因子。
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**注**：上述文献信息为示例性内容，实际研究中请通过学术数据库核实具体论文。
**Background of Recombinant Human RBM46 Protein**
RBM46 (RNA Binding Motif Protein 46) is a highly conserved RNA-binding protein belonging to the DAZ (Deleted in Azoospermia) family, characterized by its RNA recognition motifs (RRMs). Primarily expressed in germ cells, RBM46 plays a critical role in gametogenesis, particularly during spermatogenesis, where it regulates mRNA stability, translation, and splicing. It interacts with other germ cell-specific RNA-binding proteins, such as BOLL and DAZL, to coordinate post-transcriptional regulation of genes essential for germ cell development and meiosis.
Studies in animal models, including mice and zebrafish, highlight RBM46’s necessity for fertility, as its deficiency leads to germ cell arrest and sterility. In humans, RBM46 mutations or dysregulation have been linked to reproductive disorders, though its mechanisms remain under investigation. Beyond reproduction, RBM46 is implicated in certain cancers, where it may influence tumor progression through mRNA metabolism pathways.
Recombinant RBM46 protein, produced via in vitro expression systems, is pivotal for functional studies, enabling researchers to dissect its molecular interactions, RNA targets, and role in cellular processes. Its structural and functional analysis provides insights into developmental biology, fertility research, and potential therapeutic targets for related pathologies. Further research is needed to fully elucidate its regulatory networks and clinical relevance.
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