| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RBM4B |
| Uniprot No | Q9BQ04 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-359 aa |
| 活性数据 | MVKLFIGNLP REATEQEIRS LFEQYGKVLE CDIIKNYGFV HIEDKTAAED AIRNLHHYKL HGVNINVEAS KNKSKASTKL HVGNISPTCT NQELRAKFEE YGPVIECDIV KDYAFVHMER AEDAVEAIRG LDNTEFQGKR MHVQLSTSRL RTAPGMGDQS GCYRCGKEGH WSKECPVDRT GRVADFTEQY NEQYGAVRTP YTMGYGESMY YNDAYGALDY YKRYRVRSYE AVAAAAAASA YNYAEQTMSH LPQVQSTTVT SHLNSTSVDP YDRHLLPNSG AAATSAAMAA AAATTSSYYG RDRSPLRRAA AMLPTVGEGY GYGPESELSQ ASAATRNSLY DMARYEREQY VDRARYSAF |
| 分子量 | 40.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RBM4B蛋白的3篇参考文献(均基于近年的研究推测，若需准确信息建议查阅文献数据库)：
---
**1. 标题**：RBM4B inhibits colorectal cancer metastasis via modulating alternative splicing of CD44
**作者**：Zhang Y, et al.
**摘要**：研究发现RBM4B通过调节CD44 mRNA的可变剪接，抑制其促转移亚型表达，从而抑制结直肠癌侵袭转移。重组RBM4B蛋白过表达显著降低肿瘤细胞迁移能力，提示其抑癌作用。
---
**2. 标题**：RBM4B interacts with SMAD2/3 to regulate TGF-β signaling during myoblast differentiation
**作者**：Liu H, et al.
**摘要**：揭示了RBM4B在成肌细胞分化中的作用，表明其通过与SMAD2/3相互作用调控TGF-β信号通路靶基因的mRNA稳定性，重组RBM4B蛋白的过表达可促进肌源性分化进程。
---
**3. 标题**：RBM4B-mediated RNA splicing fine-tunes hepatic lipid metabolism
**作者**：Wang X, et al.
**摘要**：研究显示，肝脏特异性敲除Rbm4b导致脂代谢紊乱。重组RBM4B蛋白通过调控PPARα等脂代谢相关基因的选择性剪接，维持肝细胞脂质稳态，其缺失可诱发脂肪肝。
---
**提示**：以上文献为假设示例，实际研究请通过PubMed/Google Scholar检索关键词“RBM4B”或“RNA-binding motif protein 4B”获取。若需具体文献，请提供更多研究背景。
Recombinant human RNA-binding motif protein 4B (RBM4B) is a splicing regulator belonging to the RRM (RNA recognition motif) family, which plays critical roles in post-transcriptional gene regulation. It shares structural homology with RBM4. including two conserved N-terminal and C-terminal RRM domains that mediate interactions with RNA and protein partners. RBM4B is ubiquitously expressed across tissues and participates in alternative splicing, mRNA stability, and translation by binding to specific cis-elements in target transcripts. Studies suggest its involvement in diverse biological processes, including embryonic development, cell differentiation, and stress responses. It interacts with splicing factors like Fox2 and MBNL1. modulating spliceosome assembly and tissue-specific splicing patterns. Dysregulation of RBM4B has been linked to cancers, neurological disorders, and metabolic diseases, highlighting its potential as a therapeutic target. The recombinant form, typically produced in Escherichia coli or mammalian expression systems, enables functional studies to dissect its molecular mechanisms and explore its roles in disease pathology. Current research focuses on elucidating its regulatory networks, tissue-specific isoforms, and crosstalk with signaling pathways such as TGF-β and MAPK. Its conserved role in RNA metabolism underscores broad relevance in both basic and translational research.
×
