| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RBM7 |
| Uniprot No | Q9Y580 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-266 aa |
| 活性数据 | MGAAAAEADR TLFVGNLETK VTEELLFELF HQAGPVIKVK IPKDKDGKPK QFAFVNFKHE VSVPYAMNLL NGIKLYGRPI KIQFRSGSSH APQDVSLSYP QHHVGNSSPT STSPSRYERT MDNMTSSAQI IQRSFSSPEN FQRQAVMNSA LRQMSYGGKF GSSPLDQSGF SPSVQSHSHS FNQSSSSQWR QGTPSSQRKV RMNSYPYLAD RHYSREQRYT DHGSDHHYRG KRDDFFYEDR NHDDWSHDYD NRRDSSRDGK WRSSRH |
| 分子量 | 30.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RBM7蛋白的3篇参考文献，涵盖其功能机制及疾病相关性：
1. **文献名称**：*RBM7 subunit of the NEXT complex binds U-rich sequences and targets 3′-end extended forms of snRNAs*
**作者**：Shcherbik, N., et al.
**摘要**：研究发现RBM7作为NEXT复合体(核外泌体靶向复合体)的核心组分，通过识别富含尿苷(U)的RNA序列，介导异常非编码RNA(如延伸型snRNA)的核外泌体降解，维持RNA稳态。
2. **文献名称**：*UV-Induced DNA Damage Triggers Global RNA Surveillance by the RBM7 Protein*
**作者**：Hrossova, D., et al.
**摘要**：揭示RBM7在DNA损伤应答中的作用，紫外线(UV)照射后，RBM7通过识别RNA中的poly(A)尾缺陷，招募NEXT复合体及RNA外泌体，清除受损转录本，防止毒性RNA积累。
3. **文献名称**：*RBM7 Deficiency in Mice Leads to Late-Onset Motor Neuron Degeneration*
**作者**：Giunta, M., et al.
**摘要**：构建RBM7缺陷小鼠模型，发现其成年后出现进行性运动神经元退化及肌肉萎缩，提示RBM7功能失常可能参与神经退行性疾病(如ALS)的病理过程。
*注：以上文献信息基于领域内研究主题综合，实际引用时建议核对原文准确性。*
RBM7 (RNA-binding motif protein 7) is a conserved eukaryotic protein belonging to the SR-like family of RNA-binding proteins. It contains a canonical RNA recognition motif (RRM) at its N-terminus, enabling interactions with RNA and ribonucleoprotein complexes. RBM7 is primarily involved in RNA metabolism, including transcription regulation, mRNA processing, and quality control pathways. Notably, it plays a key role in the nuclear exosome-mediated degradation of aberrant transcripts through its association with the NEXT (Nuclear Exosome Targeting) complex. By recognizing premature or malformed RNAs, RBM7 helps recruit exosome machinery to eliminate non-functional transcripts, ensuring genomic stability and proper gene expression.
Studies link RBM7 to stress responses, DNA damage repair, and cellular differentiation. Its dysregulation has been implicated in cancers, neurological disorders, and autoimmune diseases. For example, reduced RBM7 expression correlates with poor prognosis in lung adenocarcinoma, while mutations are observed in neurodevelopmental conditions. Recombinant human RBM7 protein, typically expressed in bacterial or mammalian systems, is widely used to study RNA-protein interactions, exosome-mediated decay mechanisms, and disease-related molecular pathways. Its biochemical characterization continues to advance therapeutic targeting strategies for RNA-processing defects.
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