| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RCL1 |
| Uniprot No | Q9Y2P8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-373 aa |
| 活性数据 | MATQAYSLSYAGCNFLRQRLVLSTLSGRPVKIRKIRARDDNPGLRDFEASFIRLLDKITNGSRIEINQTGTTLYYQPGLLYGGSVEHDCSVLRGIGYYLESLLCLAPFMKHPLKIVLRGVTNDQVDPSVDVLKATALPLLKQFGIDGESFELNIVRRGMPPGGGGEVVFSCPVRKVLKPIQLTDPGKIKRIRGMAYSVRVSPQMANRIVDSARSILNKFIPDIYIYTDHMKGVNSGKSPGFGLSLVAETTSGTFLSAELASNPQGQGAAVLPEDLGRNCARLLLEEIYRGGCVDSTNQSLALLLMTLGQQDVSKVLLGPLSPYTIEFLRHLKSFFQIMFKIETKPCGEELKGGDKVLMTCVGIGFSNLSKTLK |
| 分子量 | 67.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为关于重组人RCL1蛋白的参考文献简述：
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1. **文献名称**：*Structural Insights into Human RCL1 Protein Function*
**作者**：Smith et al. (2020)
**摘要**：通过X射线晶体学解析重组人RCL1蛋白结构，揭示其在核糖体RNA前体加工中的关键作用及与酶活性相关的保守结构域。
2. **文献名称**：*RCL1 as a Novel Regulator of RNA Splicing*
**作者**：Chen & Wang (2018)
**摘要**：研究发现重组人RCL1蛋白参与RNA剪接复合体组装，调控特定mRNA剪接事件，缺失会导致细胞周期异常。
3. **文献名称**：*Recombinant RCL1 Expression and Interaction Networks*
**作者**：Garcia et al. (2021)
**摘要**：建立重组RCL1原核表达系统，结合蛋白质相互作用组学鉴定其与核糖体生物合成因子(如UTP4)的功能关联。
4. **文献名称**：*Role of RCL1 in Neurological Disorders*
**作者**：Lee et al. (2019)
**摘要**：在小脑变性小鼠模型中，重组RCL1蛋白异常表达与rRNA成熟缺陷相关，提示其在神经退行性疾病中的潜在机制。
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**注**：以上文献为虚构示例，实际引用需通过学术数据库(如PubMed或Web of Science)按具体研究方向检索真实研究。建议关注RCL1在RNA代谢、核糖体合成或疾病模型中的功能机制文献。
Recombinant human RCL1 (RNA 3'-terminal phosphate cyclase-like 1) protein is a genetically engineered version of the RCL1 enzyme, which belongs to the RNA cyclase family. RCL1 shares structural homology with RNA 3'-terminal phosphate cyclases but lacks conserved catalytic residues, suggesting distinct functional roles. It is primarily involved in ribosomal RNA (rRNA) processing and ribosome biogenesis, critical for cellular protein synthesis and growth. Studies indicate RCL1 interacts with components of the small subunit (SSU) processome, aiding in 18S rRNA maturation. Its dysfunction has been linked to developmental defects and diseases, including cancer and neurodegenerative disorders. Recombinant RCL1 is typically produced in bacterial or mammalian expression systems, enabling in vitro studies on its molecular mechanisms, interactions, and potential therapeutic applications. Research highlights its regulatory role in cell cycle progression, apoptosis, and stress responses, particularly through pathways like p53 signaling. By providing a purified, functional form of the protein, recombinant RCL1 facilitates structural analysis, enzymatic activity assays, and drug discovery efforts aimed at targeting ribosomopathies or RCL1-associated pathologies.
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