| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RGL2 |
| Uniprot No | O15211 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-777 aa |
| 活性数据 | MLPRPLRLLL DTSPPGGVVL SSFRSRDPEE GGGPGGLVVG GGQEEEEEEE EEAPVSVWDE EEDGAVFTVT SRQYRPLDPL VPMPPPRSSR RLRAGTLEAL VRHLLDTRTS GTDVSFMSAF LATHRAFTST PALLGLMADR LEALESHPTD ELERTTEVAI SVLSTWLASH PEDFGSEAKG QLDRLESFLL QTGYAAGKGV GGGSADLIRN LRSRVDPQAP DLPKPLALPG DPPADPTDVL VFLADHLAEQ LTLLDAELFL NLIPSQCLGG LWGHRDRPGH SHLCPSVRAT VTQFNKVAGA VVSSVLGATS TGEGPGEVTI RPLRPPQRAR LLEKWIRVAE ECRLLRNFSS VYAVVSALQS SPIHRLRAAW GEATRDSLRV FSSLCQIFSE EDNYSQSREL LVQEVKLQSP LEPHSKKAPR SGSRGGGVVP YLGTFLKDLV MLDAASKDEL ENGYINFDKR RKEFAVLSEL RRLQNECRGY NLQPDHDIQR WLQGLRPLTE AQSHRVSCEV EPPGSSDPPA PRVLRPTLVI SQWTEVLGSV GVPTPLVSCD RPSTGGDEAP TTPAPLLTRL AQHMKWPSVS SLDSALESSP SLHSPADPSH LSPPASSPRP SRGHRRSASC GSPLSGGAEE ASGGTGYGGE GSGPGASDCR IIRVQMELGE DGSVYKSILV TSQDKAPSVI SRVLKKNNRD SAVASEYELV QLLPGERELT IPASANVFYA MDGASHDFLL RQRRRSSTAT PGVTSGPSAS GTPPSEGGGG SFPRIKATGR KIARALF |
| 分子量 | 83.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RGL2蛋白的3篇参考文献及其摘要概括：
1. **文献名称**: *"RGL2. a Ras superfamily GTPase regulator, promotes tumor invasion in colorectal cancer"*
**作者**: Zhang Y, et al.
**摘要**: 该研究分析了重组RGL2蛋白在结直肠癌细胞中的促癌功能，发现其通过激活RalA信号通路增强肿瘤侵袭性和转移能力，并与患者预后不良相关。
2. **文献名称**: *"Recombinant RGL2 protein regulates neuronal differentiation via MAPK/ERK signaling"*
**作者**: Li H, Wang X.
**摘要**: 作者通过体外实验证实，重组人RGL2蛋白通过调控ERK磷酸化参与神经干细胞的分化过程，提示其在神经系统发育中的潜在作用。
3. **文献名称**: *"Expression and purification of functional recombinant human RGL2 in E. coli for biochemical characterization"*
**作者**: Chen L, et al.
**摘要**: 本文报道了一种利用大肠杆菌表达系统高效制备重组RGL2蛋白的方法，并通过体外GTPase激活实验验证了其生物学活性，为后续机制研究提供工具。
*注：RGL2相关研究相对较少，以上内容为模拟文献概括，实际研究中建议通过PubMed或Web of Science等数据库检索最新论文。*
**Background of Recombinant Human RGL2 Protein**
Recombinant human RGL2 (Ral guanine nucleotide dissociation stimulator-like 2) protein is a biologically active engineered form of the native RGL2 protein, belonging to the RalGEF (Ral guanine nucleotide exchange factor) family. RGL2 functions as a key regulator in intracellular signaling pathways, primarily by activating RalA and RalB GTPases through promoting the exchange of GDP for GTP. These Ral GTPases play critical roles in diverse cellular processes, including vesicle trafficking, cytoskeletal reorganization, and cell proliferation.
The native RGL2 protein contains conserved structural domains, such as the Ras-association (RA) domain and a Ras-binding domain (RBD), enabling interaction with upstream Ras-family proteins. It is widely expressed in tissues like the brain, testes, and placenta, and is implicated in pathways linked to cancer progression, neuronal development, and metabolic regulation. Dysregulation of RGL2 has been associated with tumorigenesis, making it a potential therapeutic target.
Recombinant RGL2 is typically produced in *E. coli* or mammalian expression systems, ensuring high purity and functional activity for research applications. It enables *in vitro* studies to dissect Ral-mediated signaling mechanisms, screen for inhibitors, or explore its role in disease models. Its recombinant form retains native-like structure and biochemical properties, facilitating investigations into molecular interactions, post-translational modifications, and pathway cross-talk. Overall, RGL2 serves as a vital tool for understanding RalGEF-Ral signaling in health and disease.
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