| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RFXDC1 |
| Uniprot No | Q8HWS3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-928 aa |
| 活性数据 | MAKVPKLEDTFLQAQPAPQLSPGIQEDCCVQLLGKGLLVYPEETVYLAAEGQPGGEQGGGEKGEDPELPGAVKSEMHLNNGNFSSEEEDADNHDSKTKAADQYLSQKKTITQIVKDKKKQTQLTLQWLEENYIVCEGVCLPRCILYAHYLDFCRKEKLEPACAATFGKTIRQKFPLLTTRRLGTRGHSKYHYYGIGIKESSAYYHSVYSGKGLTRFSGSKLKNEGGFTRKYSLSSKTGTLLPEFPSAQHLVYQGCISKDKVDTLIMMYKTHCQCILDNAINGNFEEIQHFLLHFWQGMPDHLLPLLENPVIIDIFCVCDSILYKVLTDVLIPATMQEMPESLLADIRNFAKNWEQWVVSSLENLPEALTDKKIPIVRRFVSSLKRQTSFLHLAQIARPALFDQHVVNSMVSDIERVDLNSIGSQALLTISGSTDTESGIYTEHDSITVFQELKDLLKKNATVEAFIEWLDTVVEQRVIKTSKQNGRSLKKRAQDFLLKWSFFGARVMHNLTLNNASSFGSFHLIRMLLDEYILLAMETQFNNDKEQELQNLLDKYMKNSDASKAAFTASPSSCFLANRNKGSMVSSDAVKNESHVETTYLPLPSSQPGGLGPALHQFPAGNTDNMPLTGQMELSQIAGHLMTPPISPAMASRGSVINQGPMAGRPPSVGPVLSAPSHCSTYPEPIYPALPQANHDFYSTSSNYQTVFRAQPHSTSGLYPHHTEHGRCMAWTEQQLSRDFFSGSCAGSPYNSRPPSSYGPSLQAQDSHNMQFLNTGSFNFLSNTGAASCQGATLPPNSPNGYYGSNINYPESHRLGSMVNQHVSVISSIRSLPPYSDIHDPLNILDDSGRKQTSSFYTDTSSPVACRTPVLASSLQTPIPSSSSQCMYGTSNQYPAQETLDSHGTSSREMVSSLPPINTVFMGTAAGGT |
| 分子量 | 128.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RFXDC1蛋白的3篇参考文献概览(注：以下内容基于模拟生成，实际文献可能需要进一步检索验证)：
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1. **标题**：*Structural insights into RFXDC1’s role in transcriptional regulation*
**作者**：Smith J, et al.
**摘要**：本研究通过重组表达纯化了人源RFXDC1蛋白，解析了其DNA结合结构域的晶体结构，揭示了其与靶基因启动子区域结合的特异性，并发现其在调节II型MHC基因中的关键作用。
2. **标题**：*RFXDC1 deficiency impairs ciliogenesis and promotes tumorigenesis in vitro*
**作者**：Wang L, et al.
**摘要**：通过构建重组RFXDC1蛋白并开展功能研究，发现其参与纤毛形成通路，缺失可导致细胞周期紊乱并增加癌细胞迁移能力，提示其在肿瘤发生中的潜在抑制功能。
3. **标题**：*Recombinant RFXDC1 as a novel biomarker for autoimmune diseases*
**作者**：Garcia R, et al.
**摘要**：开发了高纯度重组RFXDC1蛋白，用于检测患者血清中的自身抗体，实验表明其在系统性红斑狼疮(SLE)患者中表达异常，可能作为诊断标志物。
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**备注**：若需实际文献，建议通过PubMed或SciHub输入关键词“RFXDC1 recombinant”进一步筛选，可重点关注其在免疫调控、癌症及结构生物学领域的研究。
Recombinant human RFXDC1 protein is a synthesized form of the Regulatory Factor X Domain Containing 1 (RFXDC1), a member of the evolutionarily conserved RFX family of transcription factors. These proteins are characterized by a unique DNA-binding domain known as the winged-helix or X-box-binding domain, enabling recognition of specific promoter sequences (X-box motifs) to regulate gene expression. RFXDC1. though less studied compared to other RFX members like RFX1 or RFX5. is implicated in diverse biological processes, including immune regulation, ciliogenesis, and neurodevelopment. Structurally, it shares homology with other RFX proteins but lacks certain conserved regions, suggesting distinct regulatory roles.
Research indicates RFXDC1 interacts with chromatin-modifying complexes and may act as a transcriptional repressor or activator depending on cellular context. Dysregulation of RFXDC1 has been linked to cancers, such as glioblastoma and colorectal cancer, where its expression correlates with tumor progression and patient outcomes. Additionally, studies propose its involvement in immune tolerance mechanisms, potentially modulating major histocompatibility complex (MHC) class II gene expression. Recombinant RFXDC1. typically produced in Escherichia coli or mammalian systems with affinity tags (e.g., His-tag), enables functional studies, antibody development, and mechanistic exploration of its role in disease pathways. Despite emerging insights, its precise molecular targets and regulatory networks remain under investigation, highlighting the need for further research to harness its therapeutic potential.
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