| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RFX2 |
| Uniprot No | P48378 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-723 aa |
| 活性数据 | MQNSEGGADS PASVALRPSA AAPPVPASPQ RVLVQAASSN PKGAQMQPIS LPRVQQVPQQ VQPVQHVYPA QVQYVEGGDA VYTNGAIRTA YTYNPEPQMY APSSTASYFE APGGAQVTVA ASSPPAVPSH SMVGITMDVG GSPIVSSAGA YLIHGGMDST RHSLAHTSRS SPATLEMAIE NLQKSEGITS HKSGLLNSHL QWLLDNYETA EGVSLPRSSL YNHYLRHCQE HKLDPVNAAS FGKLIRSVFM GLRTRRLGTR GNSKYHYYGI RLKPDSPLNR LQEDTQYMAM RQQPMHQKPR YRPAQKTDSL GDSGSHSGLH STPEQTMAVQ SQHHQQYIDV SHVFPEFPAP DLGSFLLQDG VTLHDVKALQ LVYRRHCEAT VDVVMNLQFH YIEKLWLSFW NSKASSSDGP TSLPASDEDP EGAVLPKDKL ISLCQCDPIL RWMRSCDHIL YQALVEILIP DVLRPVPSTL TQAIRNFAKS LEGWLTNAMS DFPQQVIQTK VGVVSAFAQT LRRYTSLNHL AQAARAVLQN TSQINQMLSD LNRVDFANVQ EQASWVCQCE ESVVQRLEQD FKLTLQQQSS LDQWASWLDS VVTQVLKQHA GSPSFPKAAR QFLLKWSFYS SMVIRDLTLR SAASFGSFHL IRLLYDEYMF YLVEHRVAEA TGETPIAVMG EFNDLASLSL TLLDKDDMGD EQRGSEAGPD ARSLGEPLVK RERSDPNHSL QGI |
| 分子量 | 79.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RFX2蛋白的3篇参考文献及其摘要概述：
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1. **文献名称**: *RFX2 is essential for spermatogenesis in mice*
**作者**: Horvath J, et al.
**摘要**: 研究通过基因敲除模型揭示了RFX2在小鼠精子发生中的关键作用，证明其调控精子鞭毛相关基因的表达。重组RFX2蛋白的DNA结合实验表明其直接结合靶基因启动子区。
2. **文献名称**: *Regulation of ciliogenesis by RFX transcription factors involves direct interaction with DNAL4*
**作者**: Chung MI, et al.
**摘要**: 发现人RFX2蛋白通过结合纤毛发生相关基因的X-box启动子，调控纤毛形成。研究利用重组RFX2蛋白验证其与DNA和伴侣蛋白DNAL4的互作机制。
3. **文献名称**: *Combinatorial roles of RFX2 and RFX3 in primary cilia assembly*
**作者**: Bock T, et al.
**摘要**: 文章揭示RFX2与RFX3协同调控纤毛组装的过程，体外实验表明重组RFX2蛋白通过招募组蛋白修饰酶，激活下游纤毛结构基因的表达。
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说明：以上文献示例为虚拟生成，实际研究中建议通过PubMed或Web of Science以"RFX2 protein"、"recombinant RFX2"为关键词检索具体文献。RFX2的核心功能集中于基因转录调控，尤其在纤毛发生、生殖发育等领域。
Recombinant human RFX2 (Regulatory Factor X2) protein is a transcription factor belonging to the RFX family, which is characterized by a highly conserved DNA-binding domain known as the winged-helix or forkhead motif. RFX2 plays crucial roles in regulating gene expression by binding to X-box promoter elements, typically in collaboration with other transcriptional regulators. It is involved in diverse biological processes, including ciliogenesis, spermatogenesis, and embryonic development. Studies highlight its importance in the formation and function of motile cilia, particularly in the respiratory tract and reproductive systems. Additionally, RFX2 is implicated in neuronal differentiation and central nervous system development, with dysregulation linked to developmental disorders and infertility.
The recombinant RFX2 protein is engineered through molecular cloning, often expressed in *E. coli* or mammalian cell systems, followed by purification using affinity chromatography. Its production enables functional studies, such as DNA-binding assays, promoter activity analyses, and interaction mapping with co-regulators. Researchers utilize it to explore regulatory mechanisms in cilia-related diseases (e.g., primary ciliary dyskinesia) and reproductive health. Recombinant RFX2 also serves as a tool for screening potential therapeutic agents targeting ciliopathies or fertility issues, emphasizing its translational relevance in biomedicine.
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