| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RFPL3 |
| Uniprot No | O75679 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-317 aa |
| 活性数据 | MKRLSLVTTN RLSPQGNFLP LCTFPLAVDM AALFQEASSC PVCSDYLEKP MSLECGCTVC LKCINSLQKE PHGEDLLCCC CSMVSQRNKI RPNRQLERLV SHIKELEPKL KKILQMNPRM RKFQVDMTLD ADTANNFLLI SDDLRSVRSG LITQNRQDLA ERFDVSVCIL GSPRFTCGRH YWEVDVGTST EWDLGVCRES VHCKGKIQLT TELGFWTVSL RDGSRLSAST VPLTFLLVDR KLQRVGIFLD MGMQNVSFFD AESGSHVYTF RSVSAEEPLR PFLAPSIPPN GDQGVLSICP LMNSGTTDAP VRPGEAK |
| 分子量 | 35.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RFPL3蛋白的3篇代表性文献，按研究主题概括：
1. **文献名称**: "RFPL3 interacts with CCAR1 and regulates stem cell differentiation"
**作者**: Smith A et al.
**摘要**: 研究发现RFPL3通过结合细胞周期相关蛋白CCAR1.参与胚胎干细胞的分化调控，可能通过表观遗传修饰影响神经发育相关基因的表达。
2. **文献名称**: "Structural insights into RFPL3's role in primate-specific cortical evolution"
**作者**: Tanaka K et al.
**摘要**: 通过结构生物学分析，揭示RFPL3蛋白的锌指结构域在灵长类大脑皮层发育中的特异性功能，提示其可能推动人类高级认知能力的进化。
3. **文献名称**: "RFPL3 deficiency impairs NLRP2 ubiquitination and promotes inflammation"
**作者**: Zhou Y et al.
**摘要**: 发现RFPL3通过调控炎症小体蛋白NLRP2的泛素化降解，参与先天免疫反应，其功能异常可能与自身免疫性疾病发病相关。
注：以上为虚拟文献示例，实际研究需通过PubMed/Google Scholar等平台检索最新论文。RFPL3作为Retfinger蛋白家族成员，现有研究多聚焦于其在灵长类进化、神经发育及生殖系统调控中的独特作用。
The Recombining binding protein suppressor of hairless-like 3 (RFPL3) is a member of the RFPL gene family, which evolved through primate-specific duplication events. This family shares structural homology with the RING finger protein RBBP4/RBP1. particularly in the conserved N-terminal RING finger domain implicated in ubiquitin ligase activity or protein-protein interactions. RFPL3 is predominantly expressed in the testis and ovaries, suggesting a role in germ cell development. Studies indicate its involvement in meiotic processes, potentially regulating centriole duplication or chromatin remodeling during gametogenesis.
Notably, RFPL3 exhibits human-specific evolutionary features, including accelerated sequence divergence and unique splice variants. Epigenetic studies reveal its interaction with chromatin modifiers like KAP1. hinting at regulatory functions in gene silencing or genomic imprinting. In early embryogenesis, RFPL3 is transiently expressed during zygotic genome activation, possibly influencing totipotency transitions. However, its molecular mechanisms remain poorly characterized compared to other RFPL members. Conflicting reports exist regarding its subcellular localization (nuclear vs. cytoplasmic) and functional divergence across experimental models. Current research focuses on clarifying its interaction networks and potential roles in primate-specific developmental pathways or reproductive disorders. Further investigations are needed to establish its precise biological significance and possible clinical associations.
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