| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RFPL4B |
| Uniprot No | Q6ZWI9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-263 aa |
| 活性数据 | MAKRLQAELS CPVCLDFFSC SISLSCTHVF CFDCIQRYIL ENHDFRAMCP LCRDVVKVPA LEEWQVSVLT LMTKQHNSRL EQSLHVREEL RHFREDVTLD AATASSLLVF SNDLRSAQCK KIHHDLTKDP RLACVLGTPC FSSGQHYWEV EVGEVKSWSL GVCKEPADRK SNDLFPEHGF WISMKAGAIH ANTHLERIPA SPRLRRVGIF LDADLEEIQF FDVDNNVLIY THDGFFSLEL LCPFFCLELL GEGESGNVLT ICP |
| 分子量 | 29.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RFPL4B蛋白的模拟参考文献示例(注：文献为虚构示例，实际研究需通过学术数据库验证)：
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1. **文献名称**：*Cloning and Functional Characterization of Recombinant Human RFPL4B Protein*
**作者**：Zhang Y, et al.
**摘要**：本研究首次报道了人源RFPL4B蛋白的重组表达及纯化，通过大肠杆菌系统成功获得可溶性蛋白，并证实其在体外具有泛素连接酶活性，可能参与DNA损伤修复通路。
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2. **文献名称**：*RFPL4B Interacts with Histone H3 to Regulate Chromatin Remodeling*
**作者**：Li X, et al.
**摘要**：通过免疫共沉淀实验，作者发现重组RFPL4B蛋白与组蛋白H3直接结合，并证明其通过泛素化修饰影响染色质结构，提示其在表观遗传调控中的潜在作用。
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3. **文献名称**：*Structural Insights into the Catalytic Mechanism of RFPL4B*
**作者**：Chen L, et al.
**摘要**：该研究解析了重组RFPL4B蛋白的晶体结构(分辨率2.8Å)，揭示其RING结构域的关键氨基酸残基对底物识别的贡献，为设计靶向抑制剂提供结构基础。
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4. **文献名称**：*RFPL4B Overexpression Promotes Tumor Proliferation in Hepatocellular Carcinoma*
**作者**：Wang H, et al.
**摘要**：利用重组RFPL4B蛋白进行体外实验，发现其在肝癌细胞中异常高表达，通过激活Wnt/β-catenin信号通路促进细胞周期进程及增殖。
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**注意**：以上内容为模拟生成，实际文献需通过PubMed、Google Scholar或Web of Science等平台检索关键词“RFPL4B”或“recombinant RFPL4B”验证。
**Background of Recombinant Human RFPL4B Protein**
Recombinant human RFPL4B (Retfinger protein-like 4B) is a genetically engineered protein derived from the human *RFPL4B* gene, which belongs to the RFPL (Retfinger-like) protein family. This family is evolutionarily conserved and shares structural homology with the Retfinger protein, originally identified for its role in neuronal development. RFPL4B is primarily expressed in the brain and testis, suggesting potential functions in neurodevelopment and germ cell regulation.
Functionally, RFPL4B is proposed to act as a transcription factor or chromatin-associated protein, though its exact mechanisms remain unclear. Studies indicate its involvement in cell cycle regulation and ubiquitination processes, possibly interacting with E3 ubiquitin ligases. It is also implicated in cortical development, as knockdown experiments in model systems disrupt neuronal migration and layering.
The recombinant form of RFPL4B enables *in vitro* studies to dissect its molecular interactions, post-translational modifications, and role in diseases. Dysregulation of RFPL4B has been tentatively linked to neurodevelopmental disorders and cancers, though evidence is preliminary. Current research focuses on clarifying its biological pathways and validating its clinical relevance.
Despite progress, RFPL4B remains understudied, with many aspects of its structure, regulation, and disease associations awaiting exploration. Recombinant protein tools are critical to advancing this field.
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