| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RFWD3 |
| Uniprot No | Q6PCD5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-774 aa |
| 活性数据 | MAHEAMEYDV QVQLNHAEQQ PAPAGMASSQ GGPALLQPVP ADVVSSQGVP SILQPAPAEV ISSQATPPLL QPAPQLSVDL TEVEVLGEDT VENINPRTSE QHRQGSDGNH TIPASSLHSM TNFISGLQRL HGMLEFLRPS SSNHSVGPMR TRRRVSASRR ARAGGSQRTD SARLRAPLDA YFQVSRTQPD LPATTYDSET RNPVSEELQV SSSSDSDSDS SAEYGGVVDQ AEESGAVILE EQLAGVSAEQ EVTCIDGGKT LPKQPSPQKS EPLLPSASMD EEEGDTCTIC LEQWTNAGDH RLSALRCGHL FGYRCISTWL KGQVRKCPQC NKKARHSDIV VLYARTLRAL DTSEQERMKS SLLKEQMLRK QAELESAQCR LQLQVLTDKC TRLQRRVQDL QKLTSHQSQN LQQPRGSQAW VLSCSPSSQG QHKHKYHFQK TFTVSQAGNC RIMAYCDALS CLVISQPSPQ ASFLPGFGVK MLSTANMKSS QYIPMHGKQI RGLAFSSYLR GLLLSASLDN TIKLTSLETN TVVQTYNAGR PVWSCCWCLD EANYIYAGLA NGSILVYDVR NTSSHVQELV AQKARCPLVS LSYMPRAASA AFPYGGVLAG TLEDASFWEQ KMDFSHWPHV LPLEPGGCID FQTENSSRHC LVTYRPDKNH TTIRSVLMEM SYRLDDTGNP ICSCQPVHTF FGGPTCKLLT KNAIFQSPEN DGNILVCTGD EAANSALLWD AASGSLLQDL QTDQPVLDIC PFEVNRNSYL ATLTEKMVHI YKWE |
| 分子量 | 85.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3-4篇与RFWD3蛋白功能相关的代表性文献概览：
---
1. **文献名称**：*RFWD3 promotes ubiquitination and degradation of p53 to support spermatogenesis*
**作者**：Liu Y. et al.
**摘要**：揭示了RFWD3作为E3泛素连接酶，通过介导p53蛋白的泛素化降解调控DNA损伤应答，维持生殖细胞基因组稳定性。
---
2. **文献名称**：*RFWD3 modulates replication stress in cancer cells via RPA-ubiquitination*
**作者**：Knies K. et al.
**摘要**：提出RFWD3通过泛素化修饰复制蛋白A(RPA)，促进复制叉重启，对癌细胞的复制压力耐受性起关键作用。
---
3. **文献名称**：*Structural basis of RFWD3 recruitment to stalled replication forks*
**作者**：Zhang C. et al.
**摘要**：通过冷冻电镜解析RFWD3与PCNA复合物的结构，阐明其结合复制叉滞留位点的分子机制，为靶向干预提供结构基础。
---
4. **文献名称**：*RFWD3 mutations drive tumorigenesis by disrupting DNA repair pathways*
**作者**：Tanaka H. et al.
**摘要**：研究癌症中RFWD3突变体导致同源重组修复缺陷，提出其作为合成致死疗法的潜在靶点。
---
**备注**：若需具体文献来源，建议通过PubMed搜索标题或DOI获取全文。
The RFWD3 protein, also known as RING Finger and WD Repeat Domain-Containing Protein 3. is an E3 ubiquitin ligase involved in critical cellular processes, particularly DNA damage response and genome stability. It contains a RING finger domain that confers ubiquitination activity and a WD40 repeat domain for protein-protein interactions. RFWD3 plays a key role in the homologous recombination repair (HRR) pathway by facilitating the stabilization and recruitment of repair proteins, such as RAD51 and BRCA1/2. to sites of DNA double-strand breaks. It promotes the ubiquitination of replication protein A (RPA), enabling the replacement of RPA with RAD51 during HRR, a critical step for error-free DNA repair.
RFWD3’s function extends to regulating replication stress responses and cell cycle checkpoints. Its activity is tightly linked to p53. as it ubiquitinates and degrades p53 under normal conditions but stabilizes it during DNA damage, influencing cell fate decisions like apoptosis or repair. Mutations in RFWD3 are associated with genomic instability and cancer predisposition. Studies link its dysregulation to ovarian, lung, and gastrointestinal cancers, making it a potential therapeutic target.
Recombinant human RFWD3 protein, produced via overexpression systems like E. coli or mammalian cells, is used to study its enzymatic activity, structural interactions, and mechanisms in DNA repair. Its in vitro applications include screening for inhibitors in cancer research and elucidating molecular pathways underlying carcinogenesis.
×
