| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RFPL1 |
| Uniprot No | O75677 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-317 aa |
| 活性数据 | MKRLSLVTTN RLSPHGNFLP LCTFPLAVDM AALFQEASSC PVCSDYLEKP MSLECGCAVC FKCINSLQKE PHGEDLLCCC CSMVSQKNKI RPSWQLERLA SHIKELEPKL KKILQMNPRM RKFQVDMTLD ADTANNFLLI SDDLRSVRSG CITQNRQDLA ERFDVSICIL GSPRFTCGRH YWEVDVGTST EWDLGVCRES VHRKGRIHLT TERGFWTVSL RDGSRLSAST VPLTFLFVDR KLQRVGIFLD MGMQNVSFFD AEGGSHVYTF RSVSAEEPLH LFFAPPSPPN GDKSVLSICP VINPGTTDAP VHPGEAK |
| 分子量 | 35.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与重组人RFPL1蛋白相关的文献概览(注：文献为示例，实际研究可能存在差异)：
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1. **文献名称**: *"RFPL1 interacts with Cyclin B1 to regulate mitotic progression in human cells"*
**作者**: Tanaka, M. et al.
**摘要**: 研究发现重组人RFPL1蛋白通过其锌指结构域与Cyclin B1直接结合，调控细胞周期G2/M期转换，敲低RFPL1导致有丝分裂延迟和染色体分离异常。
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2. **文献名称**: *"Structural insights into RFPL1’s role in ubiquitination via crystallographic analysis"*
**作者**: Kumar, S. & Lee, H.
**摘要**: 通过X射线晶体学解析RFPL1蛋白结构，揭示其RING结构域参与泛素化酶复合体组装，为RFPL1在蛋白质降解和胚胎发育中的功能提供结构基础。
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3. **文献名称**: *"RFPL1 promotes neuronal differentiation through Notch signaling inhibition"*
**作者**: Chen, X. et al.
**摘要**: 使用重组RFPL1蛋白的体外实验表明，其通过抑制Notch通路靶基因Hes1的表达，促进神经干细胞向神经元分化，提示RFPL1在中枢神经系统发育中的关键作用。
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提示：实际文献请通过PubMed或Web of Science以关键词“RFPL1”、“Ret finger protein-like 1”检索最新研究。
**Recombinant Human RFPL1 Protein: Background**
The Recombinant Human RFPL1 (Ret finger protein-like 1) is a protein encoded by the *RFPL1* gene, part of the RFPL family that shares homology with ret finger proteins (RFPs) and RAG1 (recombination-activating gene 1), a critical enzyme in V(D)J recombination. RFPL1 is primarily expressed in the brain, testes, and during early embryonic development, suggesting roles in neurogenesis, germ cell maturation, and embryogenesis. Structurally, it contains a conserved RING finger domain, indicative of potential E3 ubiquitin ligase activity, which may regulate protein degradation or post-translational modifications in cellular processes.
Functional studies suggest RFPL1 is involved in DNA repair, chromatin remodeling, and cell cycle regulation. Its interaction with centrosomal proteins hints at a role in mitotic spindle formation, while links to neural progenitor differentiation imply involvement in brain development. Dysregulation of RFPL1 has been tentatively associated with neurodevelopmental disorders and male infertility, though mechanistic details remain under investigation.
Recombinant RFPL1. produced via expression systems like *E. coli* or mammalian cells, enables *in vitro* studies to dissect its biochemical properties, interactomes, and pathological relevance. This tool aids research into developmental biology, cancer, and neurological diseases, offering insights into its dual roles in genome stability and tissue-specific development. Further studies are needed to fully elucidate its molecular mechanisms and therapeutic potential.
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