| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RFFL |
| Uniprot No | Q8WZ73 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-363 aa |
| 活性数据 | MWATCCNWFC LDGQPEEVPP PQGARMQAYS NPGYSSFPSP TGLEPSCKSC GAHFANTARK QTCLDCKKNF CMTCSSQVGN GPRLCLLCQR FRATAFQREE LMKMKVKDLR DYLSLHDIST EMCREKEELV LLVLGQQPVI SQEDRTRAST LSPDFPEQQA FLTQPHSSMV PPTSPNLPSS SAQATSVPPA QVQENQQANG HVSQDQEEPV YLESVARVPA EDETQSIDSE DSFVPGRRAS LSDLTDLEDI EGLTVRQLKE ILARNFVNYK GCCEKWELME RVTRLYKDQK GLQHLVSGAE DQNGGAVPSG LEENLCKICM DSPIDCVLLE CGHMVTCTKC GKRMNECPIC RQYVIRAVHV FRS |
| 分子量 | 40.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RFFL(RING Finger and FYVE-like domain-containing protein，或RNF189)蛋白的模拟参考文献示例。由于文献检索需要实时数据库支持，建议通过PubMed或Google Scholar验证具体内容：
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1. **文献名称**: *RFFL regulates TNF-α signaling via TRAF ubiquitination in inflammatory responses*
**作者**: Zhang, Y. et al.
**摘要**: 本研究报道了重组人RFFL蛋白在HEK293细胞中的表达及其功能分析，揭示了其通过促进TRAF6的K63位泛素化激活NF-κB通路，从而调控炎症反应的作用机制。
2. **文献名称**: *Expression and structural characterization of recombinant human RFFL E3 ligase*
**作者**: Smith, J.P. et al.
**摘要**: 利用大肠杆菌系统成功表达并纯化重组人RFFL蛋白，通过晶体学解析了其RING结构域的三维结构，阐明了其介导泛素转移的关键氨基酸残基。
3. **文献名称**: *RFFL promotes EGFR degradation via ubiquitination in non-small cell lung cancer*
**作者**: Wang, L. et al.
**摘要**: 研究证实重组RFFL蛋白可通过与EGFR相互作用，诱导其泛素化降解，抑制肺癌细胞增殖，提示其作为潜在治疗靶点的可能性。
4. **文献名称**: *RFFL modulates synaptic plasticity through ubiquitin-dependent AMPAR endocytosis*
**作者**: Kim, S. et al.
**摘要**: 在神经元中表达重组RFFL蛋白，发现其通过调控AMPA受体的泛素化和内吞作用，影响突触可塑性与学习记忆功能。
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**建议**：如需准确文献，可搜索关键词 "RFFL protein recombinant" 或 "RNF189 E3 ligase" 并结合研究领域(如癌症、泛素化、炎症等)在学术数据库中进一步筛选。
Recombinant Human RFFL (RING Finger Protein Family-Like) protein is a genetically engineered protein designed to mimic the structure and function of its native counterpart. RFFL belongs to the RING finger family of E3 ubiquitin ligases, which play critical roles in ubiquitination—a post-translational modification process that regulates protein degradation, trafficking, and signaling. The RFFL protein contains a conserved RING domain responsible for binding E2 ubiquitin-conjugating enzymes, facilitating the transfer of ubiquitin molecules to target substrates. This activity is pivotal in cellular processes such as apoptosis, DNA repair, and immune response modulation.
Recombinant RFFL is typically produced using expression systems like *E. coli* or mammalian cells, ensuring high purity and bioactivity for research applications. Its study has shed light on mechanisms underlying diseases, including cancer and neurodegenerative disorders, where disrupted ubiquitination pathways contribute to pathogenesis. For instance, RFFL has been implicated in regulating TNF-α signaling and modulating the stability of oncoproteins or tumor suppressors. Researchers leverage recombinant RFFL to investigate substrate specificity, enzyme kinetics, and interactions within ubiquitination cascades. Additionally, it serves as a tool for screening therapeutic compounds targeting E3 ligases, offering potential in drug development. Despite its emerging significance, RFFL's full physiological and pathological relevance remains under exploration, highlighting its dual role as a research catalyst and therapeutic target.
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