| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RFX4 |
| Uniprot No | Q33E94 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-735 aa |
| 活性数据 | MHCGLLEEPD MDSTESWIER CLNESENKRY SSHTSLGNVS NDENEEKENN RASKPHSTPA TLQWLEENYE IAEGVCIPRS ALYMHYLDFC EKNDTQPVNA ASFGKIIRQQ FPQLTTRRLG TRGQSKYHYY GIAVKESSQY YDVMYSKKGA AWVSETGKKE VSKQTVAYSP RSKLGTLLPE FPNVKDLNLP ASLPEEKVST FIMMYRTHCQ RILDTVIRAN FDEVQSFLLH FWQGMPPHML PVLGSSTVVN IVGVCDSILY KAISGVLMPT VLQALPDSLT QVIRKFAKQL DEWLKVALHD LPENLRNIKF ELSRRFSQIL RRQTSLNHLC QASRTVIHSA DITFQMLEDW RNVDLNSITK QTLYTMEDSR DEHRKLITQL YQEFDHLLEE QSPIESYIEW LDTMVDRCVV KVAAKRQGSL KKVAQQFLLM WSCFGTRVIR DMTLHSAPSF GSFHLIHLMF DDYVLYLLES LHCQERANEL MRAMKGEGST AEVREEIILT EAAAPTPSPV PSFSPAKSAT SVEVPPPSSP VSNPSPEYTG LSTTGAMQSY TWSLTYTVTT AAGSPAENSQ QLPCMRNTHV PSSSVTHRIP VYPHREEHGY TGSYNYGSYG NQHPHPMQSQ YPALPHDTAI SGPLHYAPYH RSSAQYPFNS PTSRMEPCLM SSTPRLHPTP VTPRWPEVPS ANTCYTSPSV HSARYGNSSD MYTPLTTRRN SEYEHMQHFP GFAYINGEAS TGWAK |
| 分子量 | 83.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RFX4蛋白的模拟参考文献示例(实际文献需通过学术数据库核实)：
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1. **文献名称**: *RFX4 is a key transcriptional regulator of forebrain development*
**作者**: Blackshaw S. et al.
**摘要**: 研究揭示了RFX4在小鼠胚胎前脑发育中的关键作用，通过调控睫状体相关基因的表达维持神经祖细胞稳态，基因敲除导致严重大脑结构缺陷。
2. **文献名称**: *Recombinant RFX4 modulates TGF-β signaling in glioma cell lines*
**作者**: Zhang Y. et al.
**摘要**: 探讨了重组RFX4蛋白在胶质瘤细胞中对TGF-β信号通路的调控，证实其通过抑制Smad蛋白磷酸化影响肿瘤细胞侵袭，提示其潜在治疗价值。
3. **文献名称**: *Characterization of RFX4 isoforms in transcriptional regulation*
**作者**: Liang G. et al.
**摘要**: 分析重组RFX4不同剪接异构体的功能差异，发现特定异构体通过结合DNA保守序列调控生殖细胞发育相关基因，为不育症机制提供依据。
4. **文献名称**: *RFX4 interacts with chromatin remodelers in neural differentiation*
**作者**: Morris R. et al.
**摘要**: 研究发现重组RFX4在体外神经分化模型中与BRG1复合物协同作用，调控少突胶质细胞标志物表达，提示其在髓鞘形成中的调控网络。
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**备注**：以上为模拟示例，实际文献需通过PubMed/Google Scholar以关键词“RFX4 recombinant protein”或“RFX4 function”检索确认。
Recombinant human RFX4 (Regulatory Factor X4) protein is a transcription factor belonging to the RFX family, which regulates gene expression by binding to X-box promoter motifs. RFX4 is primarily associated with developmental processes, particularly in the central nervous system, where it plays critical roles in neural tube closure, brain patterning, and cortical development. It exists in multiple splice variants, with the full-length isoform (RFX4_v3) being essential for survival in animal models. Dysregulation of RFX4 has been implicated in neurodevelopmental disorders and cancers, including glioblastoma, where abnormal splicing may contribute to tumorigenesis.
Produced via recombinant DNA technology, RFX4 is typically expressed in bacterial (e.g., E. coli) or mammalian systems, followed by purification using affinity tags (His-tag, GST). The recombinant protein retains functional domains, including a conserved winged-helix DNA-binding domain and dimerization motifs, enabling in vitro studies on its transcriptional regulation mechanisms. Current research leverages recombinant RFX4 to investigate its interaction with co-regulators, role in ciliogenesis, and potential as a therapeutic target. Its structural and functional characterization also aids in understanding epigenetic regulation and disease-associated mutations.
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