| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RFX3 |
| Uniprot No | P48380 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-749 aa |
| 活性数据 | MQTSETGSDT GSTVTLQTSV ASQAAVPTQV VQQVPVQQQV QQVQTVQQVQ HVYPAQVQYV EGSDTVYTNG AIRTTTYPYT ETQMYSQNTG GNYFDTQGSS AQVTTVVSSH SMVGTGGIQM GVTGGQLISS SGGTYLIGNS MENSGHSVTH TTRASPATIE MAIETLQKSD GLSTHRSSLL NSHLQWLLDN YETAEGVSLP RSTLYNHYLR HCQEHKLDPV NAASFGKLIR SIFMGLRTRR LGTRGNSKYH YYGIRVKPDS PLNRLQEDMQ YMAMRQQPMQ QKQRYKPMQK VDGVADGFTG SGQQTGTSVE QTVIAQSQHH QQFLDASRAL PEFGEVEISS LPDGTTFEDI KSLQSLYREH CEAILDVVVN LQFSLIEKLW QTFWRYSPST PTDGTTITES SNLSEIESRL PKAKLITLCK HESILKWMCN CDHGMYQALV EILIPDVLRP IPSALTQAIR NFAKSLEGWL SNAMNNIPQR MIQTKVAAVS AFAQTLRRYT SLNHLAQAAR AVLQNTSQIN QMLSDLNRVD FANVQEQASW VCQCDDNMVQ RLETDFKMTL QQQSTLEQWA AWLDNVMMQA LKPYEGRPSF PKAARQFLLK WSFYSSMVIR DLTLRSAASF GSFHLIRLLY DEYMFYLVEH RVAQATGETP IAVMGEFGDL NAVSPGNLDK DEGSEVESEM DEELDDSSEP QAKREKTELS QAFPVGCMQP VLETGVQPSL LNPIHSEHIV TSTQTIRQCS ATGNTYTAV |
| 分子量 | 83.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RFX3蛋白的3篇代表性文献摘要：
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1. **文献名称**：*RFX3 controls both differentiation and elongation of multiciliated cells in the zebrafish*
**作者**：Yu et al. (2021)
**摘要**：研究通过CRISPR/Cas9技术敲除斑马鱼中的RFX3基因，发现其缺失导致多纤毛细胞分化和纤毛伸长缺陷。实验进一步利用重组人RFX3蛋白进行功能回复，证实其通过调控下游靶基因(如DNAH5)维持纤毛结构与运动功能。
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2. **文献名称**：*Regulation of ciliary motility by RFX3 via direct transcriptional activation of Dynein Axonemal genes*
**作者**：Choksi et al. (2014)
**摘要**：研究发现RFX3作为转录因子直接激活动力蛋白轴丝相关基因(如DNAH5和DNAI1)，重组人RFX3蛋白在体外实验中促进纤毛运动功能。其缺失导致小鼠胚胎纤毛运动障碍，引发脑积水等发育异常。
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3. **文献名称**：*RFX3 governs cortical neuron migration through a zinc-finger dependent mechanism*
**作者**：Benadiba et al. (2012)
**摘要**：本研究揭示RFX3通过锌指结构域调控神经元迁移相关基因表达。利用重组人RFX3蛋白体外处理小鼠脑片，部分挽救神经元迁移缺陷，表明RFX3在神经发育中的直接作用。
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**备注**：由于RFX3研究多聚焦于基因功能，明确涉及“重组人RFX3蛋白”的文献较少。上述研究或通过功能缺失/回复实验间接验证其作用，或利用重组蛋白进行机制探索，建议结合具体研究领域进一步筛选。
**Background of Recombinant Human RFX3 Protein**
The recombinant human RFX3 (Regulatory Factor X3) protein is a transcription factor belonging to the RFX family, which plays critical roles in regulating gene expression by binding to X-box promoter motifs. RFX3 is encoded by the *RFX3* gene and is structurally characterized by a highly conserved DNA-binding domain, a dimerization domain, and a transactivation domain. It is predominantly expressed in tissues such as the brain, pancreas, and ciliated epithelial cells, where it contributes to diverse biological processes, including ciliogenesis, neurodevelopment, and immune response regulation.
RFX3 is essential for the development and function of motile cilia, influencing processes like cerebrospinal fluid flow and respiratory tract clearance. In the brain, it regulates the differentiation of hypothalamic neurons and is implicated in circadian rhythm modulation. Dysregulation of RFX3 has been linked to clinical conditions such as ciliopathies, diabetes, and certain cancers. Recombinant RFX3. produced via expression systems like *E. coli* or mammalian cells, enables mechanistic studies of its role in transcriptional networks, cilia-related disorders, and signaling pathways (e.g., Hedgehog). Its applications extend to drug discovery, gene therapy research, and exploring cell differentiation pathways, offering potential therapeutic insights for diseases tied to RFX3 dysfunction.
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