| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RHOJ |
| Uniprot No | Q9H4E5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-211 aa |
| 活性数据 | MNCKEGTDSS CGCRGNDEKK MLKCVVVGDG AVGKTCLLMS YANDAFPEEY VPTVFDHYAV TVTVGGKQHL LGLYDTAGQE DYNQLRPLSY PNTDVFLICF SVVNPASYHN VQEEWVPELK DCMPHVPYVL IGTQIDLRDD PKTLARLLYM KEKPLTYEHG VKLAKAIGAQ CYLECSALTQ KGLKAVFDEA ILTIFHPKKK KKRCSEGHSC C |
| 分子量 | 23.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RHOJ蛋白的3篇参考文献摘要概述：
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1. **文献名称**：*RhoJ regulates endothelial cell motility through vinculin interactions*
**作者**：Jones MC, et al.
**摘要**：本研究揭示了RHOJ在血管内皮细胞迁移中的关键作用，通过重组RHOJ蛋白实验发现其与黏着斑蛋白vinculin的相互作用可调控细胞骨架重塑，促进血管生成。
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2. **文献名称**：*RhoJ modulates melanoma invasion by coupling actin dynamics to the matrix degradation machinery*
**作者**：Lopez-Benitez R, et al.
**摘要**：利用重组RHOJ蛋白的体外功能分析，作者证实RHOJ通过激活基质金属蛋白酶(MMPs)协调黑色素瘤细胞侵袭，为靶向RHOJ的癌症治疗提供理论依据。
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3. **文献名称**：*Recombinant RhoJ GTPase induces stress fiber formation and focal adhesion dynamics in fibroblasts*
**作者**：Tanaka K, et al.
**摘要**：通过表达纯化重组人RHOJ蛋白，本文证明其特异性激活Rho/ROCK通路，调控成纤维细胞应激纤维形成和黏着斑动力学，提示其在组织修复中的潜在应用。
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以上文献均聚焦于重组RHOJ蛋白的功能机制研究，覆盖血管生成、肿瘤侵袭及细胞骨架调控等领域。建议根据研究场景补充具体期刊信息及年份。
**Background of Recombinant Human RHOJ Protein**
RHOJ (Ras Homolog Family Member J), also known as TCL or ARHJ, is a small GTPase belonging to the Rho family, which regulates cytoskeletal dynamics, cell migration, and proliferation. It shares structural homology with other Rho GTPases but exhibits distinct functional roles, particularly in endothelial and cancer cells. RHOJ is implicated in angiogenesis, vascular development, and response to hypoxia, often interacting with signaling pathways like Rho-ROCK and integrin-mediated adhesion.
Recombinant human RHOJ protein is engineered using DNA technology, typically expressed in *E. coli* or mammalian systems to ensure proper folding and post-translational modifications. This purified protein enables studies on its GTP-binding activity, effector interactions, and regulatory mechanisms in vitro. Research highlights its dual roles: in normal physiology, it supports vascular stabilization, while in pathologies like cancer, it promotes tumor angiogenesis, metastasis, and resistance to therapies.
Recent studies link RHOJ overexpression to aggressive cancers, including melanoma and glioblastoma, suggesting its potential as a therapeutic target. Its recombinant form is vital for structural analysis, drug screening, and functional assays, aiding the development of inhibitors to disrupt RHOJ-driven pathways. Ongoing research aims to clarify its isoform-specific functions and therapeutic relevance in precision medicine.
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