| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RHOF |
| Uniprot No | Q9HBH0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-208 aa |
| 活性数据 | MDAPGALAQT AAPGPGRKEL KIVIVGDGGC GKTSLLMVYS QGSFPEHYAP SVFEKYTASV TVGSKEVTLN LYDTAGQEDY DRLRPLSYQN THLVLICYDV MNPTSYDNVL IKWFPEVTHF CRGIPMVLIG CKTDLRKDKE QLRKLRAAQL EPITYMQGLS ACEQIRAALY LECSAKFREN VEDVFREAAK VALSALKKAQ RQKKRRLC |
| 分子量 | 23.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RHOF(RhoF)蛋白的3条虚构参考文献示例，供参考：
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1. **文献名称**: "Recombinant human RhoF GTPase: Expression, purification, and functional characterization in cytoskeletal remodeling"
**作者**: Chen L, et al.
**期刊**: *Protein Expression and Purification*, 2018. Vol. 151. pp. 1-9.
**摘要**: 本研究报道了人源RHOF蛋白的重组表达及功能分析。作者通过大肠杆菌系统成功表达并纯化了具有活性的重组RHOF蛋白，并通过体外GTPase活性实验验证其酶动力学特性。实验进一步显示，RHOF可调控肌动蛋白纤维的动态组装，提示其在细胞骨架重构中的潜在作用。
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2. **文献名称**: "RhoF drives cancer cell migration by promoting filopodia formation via VASP phosphorylation"
**作者**: Tanaka K, et al.
**期刊**: *Journal of Cell Science*, 2020. Vol. 133(10), jcs.243345.
**摘要**: 该研究揭示了重组RHOF蛋白在肿瘤迁移中的作用机制。通过哺乳动物细胞表达系统获得重组RHOF，并发现其通过磷酸化VASP蛋白诱导丝状伪足形成，进而增强乳腺癌细胞的侵袭能力。研究为靶向RHOF的抗癌策略提供了理论依据。
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3. **文献名称**: "Structural insights into the GTP-binding specificity of RhoF through cryo-EM analysis"
**作者**: Müller S, et al.
**期刊**: *Nature Structural & Molecular Biology*, 2021. Vol. 28(6), pp. 512-520.
**摘要**: 本研究利用冷冻电镜解析了重组RHOF蛋白结合GTP类似物的高分辨率结构，揭示了其与经典Rho家族成员(如RhoA)的核苷酸结合口袋差异。结构分析表明，RHOF特有的柔性loop区域可能影响其下游效应蛋白的选择性。
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(注：以上文献及内容为示例性质，实际研究中请查阅真实数据库如PubMed、Web of Science获取权威信息。)
Recombinant human RHOF protein, a member of the Rho GTPase family, plays a critical role in regulating cytoskeletal dynamics and cellular processes such as migration, polarization, and morphogenesis. RHOF (Ras Homolog Family Member F) is uniquely characterized by its rapid nucleotide cycling and transient activation in response to extracellular signals. Unlike other Rho GTPases, RHOF primarily localizes to the cell periphery and membrane protrusions, where it interacts with effector proteins to modulate actin filament organization. Its involvement in stress fiber formation and focal adhesion dynamics underscores its importance in cellular motility and tissue remodeling.
Recombinant RHOF is produced using genetic engineering techniques, often in bacterial or mammalian expression systems, to enable structural and functional studies. Researchers utilize this protein to dissect signaling pathways, screen small-molecule inhibitors, and explore its therapeutic potential in pathologies like cancer metastasis, neurological disorders, and wound healing. Recent studies highlight RHOF's context-dependent roles—it can act as both a promoter and suppressor of tumor progression, depending on the microenvironment. However, challenges persist in understanding its tissue-specific regulation and interaction networks. Ongoing work aims to clarify its crosstalk with other GTPases and validate its utility as a diagnostic marker or drug target.
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