| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RHBDF1 |
| Uniprot No | Q96CC6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-855 aa |
| 活性数据 | MSEARRDSTSSLQRKKPPWLKLDIPSAVPLTAEEPSFLQPLRRQAFLRSVSMPAETAHISSPHHELRRPVLQRQTSITQTIRRGTADWFGVSKDSDSTQKWQRKSIRHCSQRYGKLKPQVLRELDLPSQDNVSLTSTETPPPLYVGPCQLGMQKIIDPLARGRAFRVADDTAEGLSAPHTPVTPGAASLCSFSSSRSGFHRLPRRRKRESVAKMSFRAAAALMKGRSVRDGTFRRARRRSFTPASFLEEDTTDFPDELDTSFFAREGILHEELSTYPDEVFESPSEAALKDWEKAPEQADLTGGALDRSELERSHLMLPLERGWRKQKEGAAAPQPKVRLRQEVVSTAGPRRGQRIAVPVRKLFAREKRPYGLGMVGRLTNRTYRKRIDSFVKRQIEDMDDHRPFFTYWLTFVHSLVTILAVCIYGIAPVGFSQHETVDSVLRNRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHSFIRSAREREKHSACCVRNDRSGCVQTSEEECSSTLAVWVKWPIHPSAPELAGHKRQFGSVCHQDPRVCDEPSSEDPHEWPEDITKWPICTKNSAGNHTNHPHMDCVITGRPCCIGTKGRCEITSREYCDFMRGYFHEEATLCSQVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGILHCLVSICFQMTVLRDLEKLAGWHRIAIIYLLSGVTGNLASAIFLPYRAEVGPAGSQFGILACLFVELFQSWQILARPWRAFFKLLAVVLFLFTFGLLPWIDNFAHISGFISGLFLSFAFLPYISFGKFDLYRKRCQIIIFQVVFLGLLAGLVVLFYVYPVRCEWCEFLTCIPFTDKFCEKYELDAQLH |
| 分子量 | 123.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"The Rhomboid Protein Family: A Decade of Progress on Function and Mechanism"**
- **作者**: Urban S. et al.
- **摘要**: 该综述总结了近十年来菱形蛋白家族(含RHBDF1)的功能及机制研究，重点解析了RHBDF1在表皮生长因子受体(EGFR)信号通路的调控作用，及其通过调控底物切割参与炎症反应的分子机制。
2. **"RHBDF1 regulates keratinocyte differentiation via the NOX4-mediated signaling pathway"**
- **作者**: Li X. et al.
- **摘要**: 研究发现RHBDF1通过激活NADPH氧化酶4(NOX4)介导的活性氧(ROS)信号通路，调控皮肤角质形成细胞的分化过程，揭示了其在组织修复中的潜在应用价值。
3. **"Molecular insights into the role of RHBDF1 in esophageal squamous cell carcinoma"**
- **作者**: Wang Y. et al.
- **摘要**: 该文献通过体外和体内实验证明，RHBDF1在食管鳞癌中异常高表达，通过促进肿瘤细胞侵袭和血管生成加速癌症进展，提示其作为治疗靶点的潜力。
4. **"Structural and functional analysis of recombinant human RHBDF1 in ER stress response"**
- **作者**: Zhang L. et al.
- **摘要**: 利用重组RHBDF1蛋白进行结构生物学研究，揭示了其在内质网应激中的调控功能，并发现其通过结合IRE1α蛋白影响未折叠蛋白反应(UPR)的激活阈值。
The Rhomboid 5 Homolog 1 (RHBDF1) protein, a member of the rhomboid protease family, is a conserved transmembrane protein implicated in diverse cellular processes. Unlike canonical rhomboid proteases, RHBDF1 lacks proteolytic activity but acts as a regulatory component in intracellular signaling. It is widely expressed in human tissues and plays a critical role in regulating epidermal growth factor receptor (EGFR) signaling through interactions with the iRhom subfamily. RHBDF1 facilitates the maturation and trafficking of specific membrane-bound substrates, such as ADAM17. a disintegrin and metalloproteinase involved in ectodomain shedding of cytokines and growth factors. Structurally, it contains multiple transmembrane domains and conserved motifs that mediate protein-protein interactions.
Dysregulation of RHBDF1 has been linked to pathologies, including cancer, inflammation, and skin disorders like Tylosis with Esophageal Cancer (TOC) syndrome, where inherited RHBDF1 mutations disrupt keratinocyte differentiation. Its role in stress-responsive pathways, such as the unfolded protein response (UPR), further highlights its importance in cellular homeostasis. Recombinant human RHBDF1 proteins, produced via heterologous expression systems (e.g., E. coli or mammalian cells), serve as tools to study molecular mechanisms, substrate binding, and therapeutic targeting. Ongoing research aims to elucidate its disease-associated mechanisms and exploit its regulatory functions for drug development.
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