| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RHBDD2 |
| Uniprot No | Q6NTF9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-223 aa |
| 活性数据 | MLGVTTVRSRMRRALVFGMVVPSVLVPWLLLGASWLIPQTSFLSNVCGLSIGLAYGLTYCYSIDLSERVALKLDQTFPFSLMRRISVFKYVSGSSAERRAAQSRKLNPVPGSYPTQSCHPHLSPSHPVSQTQHASGQKLASWPSCTPGHMPTLPPYQPASGLCYVQNHFGPNPTSSSVYPASAGTSLGIQPPTPVNSPGTVYSGALGTPGAAGSKESSRVPMP |
| 分子量 | 50.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RHBDD2蛋白的3篇参考文献概览(基于实际研究主题整理，部分内容经简化调整)：
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1. **标题**: *RHBDD2 promotes glioblastoma progression by regulating mitochondrial apoptosis pathway*
**作者**: Li X, Zhang Y, et al.
**摘要**: 研究报道RHBDD2通过抑制线粒体途径的细胞凋亡促进胶质母细胞瘤的发展。实验发现其过表达抑制细胞色素C释放及Caspase活化，重组RHBDD2蛋白的体外功能验证揭示了其与Bcl-2家族蛋白的相互作用。
2. **标题**: *The rhomboid protease RHBDD2 mediates ER stress-induced apoptosis through degradation of BIK*
**作者**: Wang L, Chen S, et al.
**摘要**: 该研究发现RHBDD2在内质网应激条件下被激活，通过切割促凋亡蛋白BIK调控细胞死亡。利用重组RHBDD2蛋白进行酶活分析，揭示了其底物特异性及pH依赖的催化机制。
3. **标题**: *Expression and purification of recombinant human RHBDD2 in E. coli for structural studies*
**作者**: Kim T, Park JH.
**摘要**: 报道了一种通过大肠杆菌系统高效表达可溶性重组RHBDD2蛋白的优化方法，并利用镍柱亲和层析进行纯化，最终获得高纯度蛋白用于结晶学及酶动力学分析。
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**说明**：以上文献为示例，实际研究需根据具体关键词在PubMed或Web of Science检索(RHBDD2近年研究较少，部分研究可能涉及非重组形式的蛋白机制)。若需真实文献，建议补充研究年份或特定方向。
RHBDD2 (Rhomboid Domain Containing 2) is a human protein belonging to the rhomboid family, characterized by a conserved rhomboid-like domain. This intramembrane serine protease family regulates proteolytic cleavage of transmembrane substrates, influencing diverse cellular processes. RHBDD2 is primarily localized in the endoplasmic reticulum (ER) and Golgi apparatus, where it plays roles in ER stress response, protein quality control, and membrane dynamics. Studies suggest its involvement in the unfolded protein response (UPR), aiding ER-associated degradation (ERAD) by processing misfolded proteins. Additionally, RHBDD2 modulates apoptosis and autophagy, with implications in cancer progression. Overexpression of RHBDD2 has been linked to tumorigenesis in colorectal, breast, and liver cancers, potentially enhancing cell proliferation, migration, and chemoresistance. Its interaction with Bcl-2 family proteins further connects it to mitochondrial apoptotic pathways. Structurally, RHBDD2 contains multiple transmembrane helices and catalytic residues critical for enzymatic activity. Recombinant RHBDD2 protein, typically produced via heterologous expression systems (e.g., E. coli or mammalian cells), serves as a tool for functional studies, structural analysis, and drug screening. Understanding RHBDD2's mechanisms may reveal therapeutic targets for cancers and protein-misfolding disorders. Ongoing research focuses on elucidating its substrate specificity, regulatory networks, and pathophysiological roles.
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