| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Rgr |
| Uniprot No | P47804 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 0 |
| 活性数据 | MAETSALPTGFGELEVLAVGMVLLVEALSGLSLNTLTIFSFCKTPELRTPCHLLVLSLALADSGISLNALVAATSSLLRRWPYGSDGCQAHGFQGFVTALASICSSAAIAWGRYHHYCTRSQLAWNSAVSLVLFVWLSSAFWAALPLLGWGHYDYEPLGTCCTLDYSKGDRNFTSFLFTMSFFNFAMPLFITITSYSLMEQKLGKSGHLQVNTTLPARTLLLGWGPYAILYLYAVIADVTSISPKLQMVPALIAKMVPTINAINYALGNEMVCRGIWQCLSPQKREKDRTK |
| 分子量 | 31.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RGR蛋白的参考文献示例(注：部分文献内容为示例性概括，实际引用需核实原文)：
1. **文献名称**：*"Cloning and Functional Expression of the Human Retinal G Protein-Coupled Receptor (RGR)"*
**作者**：Chen P et al.
**摘要**：本研究成功克隆了人源RGR基因，并在哺乳动物细胞中实现了重组表达，揭示了RGR在视黄醇代谢中的光依赖性激活机制，为研究其在视网膜疾病中的作用提供了模型。
2. **文献名称**：*"Recombinant Human RGR Protein: Purification and Structural Characterization"*
**作者**：Kim S et al.
**摘要**：报道了重组RGR蛋白在大肠杆菌中的高效表达与纯化流程，并通过圆二色谱(CD)分析其二级结构，证实其具有典型的G蛋白偶联受体折叠特征。
3. **文献名称**：*"RGR-Opsin Interactions in Retinoid Recycling: Insights from Recombinant Protein Studies"*
**作者**：Rossi M et al.
**摘要**：利用重组RGR蛋白与视蛋白共表达系统，阐明了RGR在视觉周期中促进11-顺式视黄醇再生的重要功能，揭示了其与光受体蛋白的分子互作机制。
4. **文献名称**：*"Targeted Delivery of Recombinant RGR for Retinal Degeneration Therapy"*
**作者**：Li X et al.
**摘要**：开发了一种基于重组RGR蛋白的纳米载体递送系统，实验证明其能有效改善视网膜色素上皮细胞的功能缺陷，为治疗年龄相关性黄斑变性提供新策略。
**注意**：以上文献信息为示例性内容，实际研究需通过学术数据库(如PubMed、Web of Science)检索并核实原文准确性。
Recombinant human RGR (Retinal G protein-coupled receptor) protein is a genetically engineered form of the native RGR protein, primarily expressed in retinal pigment epithelial (RPE) cells and Müller glia. RGR belongs to the opsin subfamily of G protein-coupled receptors (GPCRs) and plays a critical role in the visual cycle. Unlike classical visual opsins, RGR functions as a retinoid isomerase, facilitating the light-dependent conversion of all-trans-retinol to 11-cis-retinol, a key step in regenerating visual pigments for phototransduction. This activity is essential for maintaining retinal homeostasis and visual function under varying light conditions.
The recombinant RGR protein is typically produced in heterologous expression systems (e.g., mammalian cells or insect cells) to ensure proper post-translational modifications and membrane protein folding. Its study has provided insights into degenerative retinal diseases, such as retinitis pigmentosa and age-related macular degeneration, where disrupted retinoid metabolism is implicated. Researchers utilize recombinant RGR to investigate molecular mechanisms of vitamin A recycling, screen therapeutic compounds, and develop gene therapies. Challenges in production include optimizing stability and solubility for structural studies (e.g., X-ray crystallography) and functional assays.
Recent advances highlight RGR's potential as a therapeutic target or biomarker for retinal disorders. Further research aims to unravel its interactions with retinoid-binding proteins and regulatory pathways in both physiological and disease contexts.
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