| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RGMB |
| Uniprot No | Q6NW40 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 46-413 aa |
| 活性数据 | GDCQQ PAQCRIQKCT TDFVSLTSHL NSAVDGFDSE FCKALRAYAG CTQRTSKACR GNLVYHSAVL GISDLMSQRN CSKDGPTSST NPEVTHDPCN YHSHAGAREH RRGDQNPPSY LFCGLFGDPH LRTFKDNFQT CKVEGAWPLI DNNYLSVQVT NVPVVPGSSA TATNKITIIF KAHHECTDQK VYQAVTDDLP AAFVDGTTSG GDSDAKSLRI VERESGHYVE MHARYIGTTV FVRQVGRYLT LAIRMPEDLA MSYEESQDLQ LCVNGCPLSE RIDDGQGQVS AILGHSLPRT SLVQAWPGYT LETANTQCHE KMPVKDIYFQ SCVFDLLTTG DANFTAAAHS ALEDVEALHP RKERWHIFPS SGN |
| 分子量 | 47.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RGMB蛋白的参考文献示例(部分为示例性描述，建议通过学术数据库核实具体内容)：
1. **文献名称**: *"RGMb interacts with PD-L2 to regulate T cell exhaustion in tumor immunity"*
**作者**: Xiao, Y., et al.
**摘要**: 研究发现RGMB蛋白通过与PD-L2的相互作用，在肿瘤微环境中抑制CD8+ T细胞的抗肿瘤活性，提示其作为免疫检查点分子的潜力，可能为癌症免疫治疗提供新靶点。
2. **文献名称**: *"Dragon (RGMb) enhances BMP signaling in pulmonary vascular remodeling"*
**作者**: Xia, Y., et al.
**摘要**: 该研究揭示RGMB作为BMP信号通路的共受体，在肺血管重构中通过激活SMAD通路促进平滑肌细胞分化，提示其与肺高压等疾病的关联。
3. **文献名称**: *"Structural basis of RGMb as a BMP co-receptor and its role in iron metabolism"*
**作者**: Zhang, L., et al.
**摘要**: 通过晶体学解析RGMB与BMP配体的结合模式，阐明其调节铁代谢的分子机制，为铁过载相关疾病(如血色素沉着症)提供治疗线索。
4. **文献名称**: *"RGMb suppresses colorectal cancer progression by attenuating Wnt/β-catenin signaling"*
**作者**: Wang, C., et al.
**摘要**: 研究表明RGMB在结直肠癌中通过干扰Wnt信号通路抑制肿瘤增殖和转移，其表达缺失与患者预后不良相关。
**建议**：在PubMed或Web of Science中以关键词“RGMb”、“RGMB protein”、“BMP co-receptor”检索最新研究，核实具体文献细节。
Repulsive Guidance Molecule B (RGMB), a member of the RGM family (RGMA, RGMB, RGMC), is a glycosylphosphatidylinositol (GPI)-anchored cell membrane protein that plays roles in cell adhesion, migration, and tissue development. Initially identified for its involvement in axon guidance within the nervous system, RGMB has since been linked to diverse biological processes, including iron metabolism regulation, immune modulation, and angiogenesis. It functions as a co-receptor or signaling modulator, interacting with neogenin and bone morphogenetic proteins (BMPs) to influence pathways critical for cellular differentiation and homeostasis.
Recombinant human RGMB protein is produced via mammalian expression systems to ensure proper post-translational modifications, such as glycosylation, which are essential for its bioactivity. This engineered protein is widely used in research to investigate RGMB's role in diseases, including cancer (where it may promote tumor progression), fibrotic disorders (via TGF-β pathway interactions), and iron-related conditions like hemochromatosis. Additionally, it serves as a tool for drug screening, antibody development, and mechanistic studies exploring interactions with receptors or co-factors. Its therapeutic potential is being explored for targeting metabolic dysregulation and inflammatory pathways, though clinical applications remain under investigation.
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