| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RHBDL3 |
| Uniprot No | P58872 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-306 aa |
| 活性数据 | MSNKRSNSFRQAILQGNRRLSSKALLEEKGLSLSQRLIRHVAYETLPREIDRKWYYDSYTCCPPPWFMITVTLLEVAFFLYNGVSLGQFVLQVTHPRYLKNSLVYHPQLRAQVWRYLTYIFMHAGIEHLGLNVVLQLLVGVPLEMVHGATRIGLVYVAGVVAGSLAVSVADMTAPVVGSSGGVYALVSAHLANIVMNWSGMKCQFKLLRMAVALICMSMEFGRAVWLRFHPSAYPPCPHPSFVAHLGGVAVGITLGVVVLRNYEQRLQDQSLWWIFVAMYTVFVLFAVFWNIFAYTLLDLKLPPPP |
| 分子量 | 60.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RHBDL3蛋白的3篇代表性文献(均为虚拟示例，实际文献需通过学术数据库检索获取)：
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1. **文献名称**：*RHBDL3 promotes EGFR signaling through cleavage of EGF-like ligands*
**作者**：Smith A et al.
**摘要**：研究揭示了RHBDL3作为膜内蛋白酶通过切割表皮生长因子(EGF)家族配体，激活EGFR信号通路，促进细胞增殖和迁移的分子机制，暗示其在癌症发展中的潜在作用。
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2. **文献名称**：*Structural basis of RHBDL3 substrate selectivity and rhomboid protease evolution*
**作者**：Chen L, Zhang Q
**摘要**：通过解析RHBDL3的晶体结构，阐明了其底物识别特异性的结构基础，并对比不同物种菱形蛋白酶的进化差异，提出其功能多样化的关键氨基酸位点。
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3. **文献名称**：*RHBDL3 regulates synaptic plasticity via cleavage of neuroligin-1*
**作者**：Wang Y et al.
**摘要**：发现RHBDL3在中枢神经系统中特异性切割突触黏附分子neuroligin-1.调控突触可塑性和神经元网络形成，为神经退行性疾病机制提供了新视角。
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如需获取真实文献，建议在PubMed或Web of Science中检索关键词：**RHBDL3 OR Rhomboid-like 3 protease**，筛选近年高被引或权威期刊论文。
Rhomboid-like protein 3 (RHBDL3), a member of the rhomboid protease family, is an intramembrane serine protease conserved across eukaryotes. Unlike most rhomboid proteases localized to mitochondria or secretory pathways, RHBDL3 is primarily found in the plasma membrane and Golgi apparatus, where it plays roles in regulating cell surface protein shedding and intracellular signaling. It features a characteristic six-transmembrane domain structure with a catalytic serine-histidine dyad embedded within the lipid bilayer, enabling proteolysis of transmembrane substrates without requiring prior endocytosis.
RHBDL3 is implicated in diverse physiological processes, including extracellular matrix remodeling, receptor signaling, and cellular adhesion. A key substrate is the amyloid precursor protein (APP), which RHBDL3 cleaves to modulate neurotoxic Aβ peptide production, potentially linking it to Alzheimer's disease pathways. Emerging studies also associate RHBDL3 with cancer progression through cleavage of adhesion molecules like ephrin-B3. influencing tumor metastasis.
Despite functional insights, many aspects remain unclear, including its precise substrate spectrum and regulatory mechanisms. Recent structural studies using cryo-EM have revealed auto-inhibitory conformations, suggesting tight spatial control of its protease activity. RHBDL3's dual role in neurological disorders and oncogenesis positions it as a compelling therapeutic target, driving ongoing research into selective inhibitors and biological probes to dissect its pathophysiological contributions.
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