| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RKHD1 |
| Uniprot No | Q86XN8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-651 aa |
| 活性数据 | MPSSLGQPDG GGGGGGGGGG VGAAGEDPGP GPAPPPEGAQ EAAPAPRPPP EPDDAAAALR LALDQLSALG LGGAGDTDEE GAAGDGAAAA GGADGGAAPE PVPPDGPEAG APPTLAPAVA PGSLPLLDPN ASPPPPPPPR PSPPDVFAGF APHPAALGPP TLLADQMSVI GSRKKSVNMT ECVPVPSSEH VAEIVGRQGC KIKALRAKTN TYIKTPVRGE EPVFIVTGRK EDVEMAKREI LSAAEHFSII RATRSKAGGL PGAAQGPPNL PGQTTIQVRV PYRVVGLVVG PKGATIKRIQ QRTHTYIVTP GRDKEPVFAV TGMPENVDRA REEIEAHITL RTGAFTDAGP DSDFHANGTD VCLDLLGAAA SLWAKTPNQG RRPPTATAGL RGDTALGAPS APEAFYAGSR GGPSVPDPGP ASPYSGSGNG GFAFGAEGPG APVGTAAPDD CDFGFDFDFL ALDLTVPAAA TIWAPFERAA PLPAFSGCST VNGAPGPPAA GARRSSGAGT PRHSPTLPEP GGLRLELPLS RRGAPDPVGA LSWRPPQGPV SFPGGAAFST ATSLPSSPAA AACAPLDSGA SENSRKPPSA SSAPALAREC VVCAEGEVMA ALVPCGHNLF CMDCAVRICG KSEPECPACR TPATQAIHIF S |
| 分子量 | 64.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RKHD1蛋白的模拟参考文献(非真实文献，供参考)：
1. **文献名称**：Structure and Functional Analysis of Recombinant Human RKHD1 in TGF-β Signaling
**作者**：Li X, et al.
**摘要**：研究利用大肠杆菌系统表达重组人RKHD1蛋白，发现其通过C端结构域与Smad4相互作用，调控TGF-β信号通路中的细胞迁移。
2. **文献名称**：RKHD1 Knockdown Inhibits Tumor Angiogenesis via VEGF Pathway
**作者**：Wang Y, et al.
**摘要**：通过构建RKHD1重组蛋白及功能实验，证明其在癌细胞中通过结合VEGF受体促进血管生成，靶向RKHD1可抑制小鼠移植瘤生长。
3. **文献名称**：Expression and Purification of Recombinant RKHD1 for Antibody Development
**作者**：Chen L, et al.
**摘要**：优化昆虫细胞系统中RKHD1的分泌表达，纯化后作为抗原制备单克隆抗体，用于肝癌患者样本中RKHD1的高灵敏度检测。
4. **文献名称**：RKHD1 Interaction with MAPK Cascade in Neuronal Differentiation
**作者**：Garcia-Rodriguez F, et al.
**摘要**：重组RKHD1蛋白体外实验表明其通过磷酸化ERK1/2参与神经干细胞分化，提示其在神经发育疾病中的潜在作用。
**说明**：以上文献为模拟示例，RKHD1相关研究需通过PubMed/Google Scholar检索确认。建议用户核实蛋白名称准确性(例如是否为Rho-Kinase结构域相关蛋白或其他基因别名)。
Recombinant human RKHD1 (Radial spoke head domain-containing protein 1) is a genetically engineered protein derived from the human RKHD1 gene. This protein is characterized by a conserved radial spoke head domain, which is structurally homologous to components found in the axonemal radial spokes of cilia and flagella. These structures are critical for regulating motility and sensory functions in eukaryotic cells. RKHD1 is hypothesized to play a role in cellular processes involving cilia, such as intracellular signaling, cell cycle regulation, or mechanical force transduction. Dysregulation of ciliary proteins, including RKHD1. has been linked to ciliopathies, a group of disorders affecting multiple organ systems.
The recombinant form is typically produced in heterologous expression systems (e.g., E. coli, mammalian cells) to enable functional studies, structural analysis, and antibody development. Its production allows researchers to investigate interactions with binding partners, enzymatic activities, or involvement in pathways like Hedgehog signaling, which relies on primary cilia. Current research focuses on elucidating its physiological role and potential therapeutic applications, particularly in diseases tied to ciliary dysfunction. Despite progress, the exact molecular mechanisms and full spectrum of RKHD1’s biological functions remain under exploration.
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