| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RIOK2 |
| Uniprot No | Q9BVS4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-552 aa |
| 活性数据 | MGKVNVAKLR YMSRDDFRVL TAVEMGMKNH EIVPGSLIAS IASLKHGGCN KVLRELVKHK LIAWERTKTV QGYRLTNAGY DYLALKTLSS RQVVESVGNQ MGVGKESDIY IVANEEGQQF ALKLHRLGRT SFRNLKNKRD YHKHRHNVSW LYLSRLSAMK EFAYMKALYE RKFPVPKPID YNRHAVVMEL INGYPLCQIH HVEDPASVYD EAMELIVKLA NHGLIHGDFN EFNLILDESD HITMIDFPQM VSTSHPNAEW YFDRDVKCIK DFFMKRFSYE SELFPTFKDI RREDTLDVEV SASGYTKEMQ ADDELLHPLG PDDKNIETKE GSEFSFSDGE VAEKAEVYGS ENESERNCLE ESEGCYCRSS GDPEQIKEDS LSEESADARS FEMTEFNQAL EEIKGQVVEN NSVTEFSEEK NRTENYNRQD GQRVQGGVPA GSDEYEDECP HLIALSSLNR EFRPFRDEEN VGAMNQYRTR TLSITSSGSA VSCSTIPPEL VKQKVKRQLT KQQKSAVRRR LQKGEANIFT KQRRENMQNI KSSLEAASFW GE |
| 分子量 | 63.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RIOK2蛋白的3篇代表性参考文献的简要列举(注：文献信息为虚拟示例，实际研究需参考真实数据库)：
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1. **"Recombinant human RIOK2 kinase: Purification and structural insights into its atypical ATP-binding mechanism"**
- **作者**: Zhang L, et al.
- **摘要**: 该研究通过大肠杆菌系统成功表达并纯化了重组人RIOK2蛋白，利用X射线晶体学解析其三维结构，揭示了RIOK2非典型ATP结合域的特性，并探讨其激酶活性的调控机制。
2. **"RIOK2 promotes tumor metastasis via Wnt/β-catenin signaling in triple-negative breast cancer"**
- **作者**: Chen Y, Wang H, Li J.
- **摘要**: 研究通过重组RIOK2蛋白的过表达模型，证明其在三阴性乳腺癌中通过磷酸化关键底物激活Wnt/β-catenin通路，促进肿瘤转移，为靶向RIOK2的疗法提供了依据。
3. **"Functional characterization of RIOK2 in ribosome biogenesis using CRISPR and recombinant protein complementation"**
- **作者**: Müller R, et al.
- **摘要**: 结合CRISPR敲除技术和重组RIOK2蛋白回补实验，发现RIOK2在核糖体RNA加工中起关键作用，其缺失导致细胞周期停滞并影响核仁结构完整性。
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如需真实文献，建议在PubMed或Web of Science中检索关键词(如"recombinant RIOK2"、"RIOK2 protein function")，并按时间排序筛选近年研究。
RIO kinase 2 (RIOK2) is a member of the evolutionarily conserved RIO serine/threonine kinase family, characterized by a unique kinase domain distinct from classical protein kinases. In humans, RIOK2 plays critical roles in cell cycle progression, ribosome biogenesis, and signal transduction pathways. It is ubiquitously expressed and localizes predominantly to the cytoplasm. Functionally, RIOK2 interacts with multiple signaling cascades, including RAS/MAPK and mTOR pathways, influencing cell proliferation, survival, and differentiation. Studies link RIOK2 to cancer biology, where its overexpression correlates with poor prognosis in malignancies like glioblastoma, leukemia, and colorectal cancer. Mechanistically, RIOK2 modulates pre-ribosomal RNA processing and maturation, bridging kinase activity to translational regulation. Recent evidence also implicates RIOK2 in innate immune responses through its interaction with antiviral signaling proteins. As an atypical kinase with auto-phosphorylation capacity but limited substrate diversity, RIOK2’s catalytic mechanisms remain under investigation. Recombinant human RIOK2 protein, typically produced in *E. coli* or mammalian expression systems, serves as a vital tool for structural studies, enzymatic assays, and inhibitor screening. Its conserved nature across eukaryotes makes it a model for studying kinase evolution and ribosome-related pathologies. Therapeutic targeting of RIOK2 is emerging as a potential strategy for cancer and immune-related disorders.
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