| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RING1 |
| Uniprot No | Q8N488 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-228 aa |
| 活性数据 | MTMGDKKSPT RPKRQAKPAA DEGFWDCSVC TFRNSAEAFK CSICDVRKGT STRKPRINSQ LVAQQVAQQY ATPPPPKKEK KEKVEKQDKE KPEKDKEISP SVTKKNTNKK TKPKSDILKD PPSEANSIQS ANATTKTSET NHTSRPRLKN VDRSTAQQLA VTVGNVTVII TDFKEKTRSS STSSSTVTSS AGSEQQNQSS SGSESTDKGS SRSSTPKGDM SAVNDESF |
| 分子量 | 24.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人RING1蛋白相关的参考文献示例(文献信息为模拟示例，仅供参考)：
1. **《RING1 interacts with multiple Polycomb-group proteins and mediates transcriptional repression》**
- 作者：Satijn, D.P., et al.
- 摘要：本文首次证明RING1蛋白是Polycomb抑制复合物PRC1的核心成员，通过与Bmi1等其他Polycomb蛋白相互作用参与基因沉默，并通过泛素化组蛋白H2A(H2AK119ub)调控靶基因的染色质修饰。
2. **《Role of RING1B in the dynamic regulation of developmental gene expression》**
- 作者：Endoh, M., et al.
- 摘要：研究揭示了RING1B(RING1的同源蛋白)在胚胎干细胞分化中的作用，通过PRC1复合物催化H2AK119泛素化，沉默发育相关基因，维持细胞多能性及分化时序调控。
3. **《Structural basis of RING1 E3 ubiquitin ligase activity in Polycomb gene silencing》**
- 作者：Buchwald, G., et al.
- 摘要：通过结构生物学分析，阐明RING1蛋白的RING结构域如何与E2泛素结合酶协同作用，催化组蛋白H2A的泛素化，为Polycomb介导的表观遗传调控提供分子机制解释。
4. **《RING1 and Bmi1 cooperate in chromatin remodeling and cancer progression》**
- 作者：Wang, H., et al.
- 摘要：研究发现RING1与Bmi1在PRC1复合物中协同作用，通过异常沉默抑癌基因调控细胞周期，促进多种癌症的进展，为靶向Polycomb通路的治疗策略提供依据。
(注：以上文献标题及作者为学术假设，建议通过PubMed或Web of Science以“RING1 protein”、“PRC1 complex”等关键词检索真实文献。)
Recombinant human RING1 protein, also known as RING1A or RING1B, is a critical component of the Polycomb Repressive Complex 1 (PRC1), which plays a central role in epigenetic gene silencing. RING1 proteins are E3 ubiquitin ligases that catalyze monoubiquitination of histone H2A at lysine 119 (H2AK119ub), a hallmark of transcriptionally repressive chromatin. This post-translational modification facilitates chromatin compaction and recruitment of additional silencing factors, maintaining stable gene repression during development and cellular differentiation.
RING1 proteins interact with other Polycomb group (PcG) proteins, such as BMI1 and PHC, to regulate stem cell pluripotency, embryogenesis, and cell cycle progression. Dysregulation of RING1 function is implicated in cancers, developmental disorders, and neurodegenerative diseases. Recombinant RING1 is commonly produced in bacterial or eukaryotic expression systems for biochemical and structural studies, enabling researchers to dissect its enzymatic activity, protein interactions, and regulatory mechanisms in vitro.
Its recombinant form has become a valuable tool for investigating PRC1-mediated gene regulation, screening inhibitors for therapeutic development, and modeling epigenetic defects in diseases. The protein’s modular structure, featuring a conserved RING domain essential for E2 ubiquitin-conjugating enzyme binding, underpins its functional versatility in chromatin remodeling pathways.
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