| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RIN2 |
| Uniprot No | Q8WYP3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 786-894 aa |
| 活性数据 | DFQNYLRVAFQEVNSGCTGKTLLVRPYITTEDVCQICAEKFKVGDPEEYSLFLFVDETWQQLAEDTYPQKIKAELHSRPQPHIFHFVYKRIKNDPYGIIFQNGEEDLTT |
| 分子量 | 37.73 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RIN2蛋白的3篇假设性参考文献示例，基于典型研究方向的推测归纳：
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1. **文献名称**：*Expression and Functional Characterization of Recombinant Human RIN2 Protein in Eukaryotic Cells*
**作者**：Smith J, et al.
**摘要**：本研究成功在HEK293细胞中表达了重组人RIN2蛋白，并通过免疫沉淀和共聚焦显微技术证实其与Rab5 GTP酶的相互作用，揭示了RIN2在调控内体膜运输中的关键作用。
2. **文献名称**：*Structural Insights into RIN2-mediated Signaling Pathways via Recombinant Protein Analysis*
**作者**：Zhang L, et al.
**摘要**：利用重组RIN2蛋白进行体外结合实验，结合质谱分析，鉴定了RIN2与生长因子受体(如EGFR)的相互作用界面，提出其作为衔接蛋白在Ras/MAPK信号通路中的调控机制。
3. **文献名称**：*Disease-associated Mutations in RIN2 Disrupt Recombinant Protein Stability and Rab5 Binding*
**作者**：Tanaka K, et al.
**摘要**：通过构建携带RIN2综合征相关突变体(如p.R312C)的重组蛋白，发现突变导致蛋白稳定性下降及Rab5结合能力减弱，解释了患者细胞中内体运输障碍的分子基础。
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**说明**：以上文献为示例，实际研究中需以真实发表的论文为准。建议通过PubMed、Web of Science等平台，以“RIN2 recombinant protein”、“RIN2 Rab5 interaction”或“RIN2 syndrome”等关键词检索最新文献。
**Background of RIN2 Protein**
RIN2 (Ras and Rab interactor 2) is a multifunctional adaptor protein belonging to the RIN family, known for its role in intracellular signaling and membrane trafficking. It contains conserved structural domains, including a Ras-association (RA) domain, a proline-rich region with SH3-binding motifs, and a VPS9 domain responsible for guanine nucleotide exchange factor (GEF) activity toward Rab5 and Rab31 GTPases. These features enable RIN2 to regulate endocytic trafficking, cytoskeletal reorganization, and receptor signaling.
RIN2 interacts with Rab5 and Rab31 to mediate endosome maturation and recycling, influencing cellular processes like growth factor signaling and adhesion. Dysregulation of RIN2 is linked to pathological conditions. Biallelic mutations in the *RIN2* gene cause RIN2 syndrome, a rare autosomal recessive disorder characterized by facial dysmorphism, joint hyperlaxity, skin fragility, and skeletal abnormalities. Studies suggest RIN2’s involvement in connective tissue homeostasis and neural development, though its exact mechanisms remain under investigation.
Research on RIN2 highlights its dual role as a molecular scaffold and Rab activator, bridging Ras/Rab signaling pathways with cellular dynamics. Understanding RIN2’s functions may provide insights into trafficking-related disorders and potential therapeutic strategies. Current investigations focus on elucidating its interactome and tissue-specific roles in health and disease. (Word count: 248)
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