| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RILPL2 |
| Uniprot No | Q969X0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-211 aa |
| 活性数据 | MEEPPVREEE EEEGEEDEER DEVGPEGALG KSPFQLTAED VYDISYLLGR ELMALGSDPR VTQLQFKVVR VLEMLEALVN EGSLALEELK MERDHLRKEV EGLRRQSPPA SGEVNLGPNK MVVDLTDPNR PRFTLQELRD VLQERNKLKS QLLVVQEELQ CYKSGLIPPR EGPGGRREKD AVVTSAKNAG RNKEEKTIIK KLFFFRSGKQ T |
| 分子量 | 23.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于假设的与RILPL2蛋白相关的参考文献示例。需注意，由于RILPL2的研究相对有限，部分内容可能需要根据实际文献调整：
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1. **文献名称**：*RILPL2 regulates ciliogenesis by recruiting Tau tubulin kinase 2 to the basal body*
**作者**：Smith A, et al.
**摘要**：本研究揭示RILPL2通过结合微管相关蛋白Tau tubulin kinase 2(TTBK2)，调控纤毛形成过程。实验显示RILPL2缺失导致初级纤毛结构异常，提示其在细胞极性及信号转导中的关键作用。
2. **文献名称**：*Interaction of RILPL2 with Rab small GTPases in vesicular trafficking*
**作者**：Chen L, et al.
**摘要**：发现RILPL2与Rab8和Rab10相互作用，参与细胞内膜运输及细胞迁移。敲除RILPL2会破坏细胞囊泡运输，表明其在细胞内运输通路中的调控功能。
3. **文献名称**：*RILPL2 overexpression promotes cancer cell invasion via ERK signaling*
**作者**：Tanaka K, et al.
**摘要**：在乳腺癌模型中，RILPL2高表达通过激活ERK/MAPK通路增强细胞侵袭能力，提示其可能作为肿瘤转移的潜在标志物。
4. **文献名称**：*Structural analysis of RILPL2 reveals its role in centriole duplication*
**作者**：Zhang Y, et al.
**摘要**：通过冷冻电镜解析RILPL2结构，发现其与中心体蛋白Cep68结合，调控中心体复制及细胞周期进程。
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**注意事项**：
- 上述文献为假设示例，实际研究可能需查阅PubMed/Google Scholar等数据库(关键词：RILPL2、ciliogenesis、Rab GTPases、cancer)。
- 若研究较少，可扩展至RILPL2同源蛋白(如RILPL1)或相关通路(纤毛发生、细胞迁移)。
- 推荐使用Zotero/EndNote等工具管理参考文献格式(如APA/MLA)。
**Background of Recombinant Human RILPL2 Protein**
Recombinant human RILPL2 (Rab-interacting lysosomal protein-like 2) is a protein encoded by the *RILPL2* gene, belonging to the RILP (Rab-interacting lysosomal protein) family. Structurally, RILPL2 contains N-terminal coiled-coil domains and a C-terminal Rab-binding domain, enabling interactions with small GTPases of the Rab family, particularly Rab8 and Rab10. These interactions position RILPL2 as a key regulator of intracellular trafficking, cytoskeletal dynamics, and organelle positioning.
RILPL2 is notably involved in ciliogenesis, the process of forming primary cilia—an antenna-like organelle critical for sensory and signaling functions in eukaryotic cells. By modulating Rab8/Rab10 activity, RILPL2 facilitates vesicle transport and membrane docking required for ciliary assembly. Additionally, it plays roles in mitotic progression by influencing centrosome positioning and spindle orientation, ensuring proper cell division. Dysregulation of RILPL2 has been linked to ciliopathies (e.g., polycystic kidney disease, retinal degeneration) and cancer, where aberrant expression correlates with tumor invasiveness and poor prognosis.
Recombinant RILPL2 proteins are widely used to study Rab-mediated pathways, cilia-related disorders, and cancer mechanisms. They also serve as tools for drug screening aimed at targeting ciliogenesis or Rab-dependent processes. Research continues to explore its dual roles in cellular homeostasis and disease, highlighting its potential as a therapeutic or diagnostic biomarker.
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