| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RICS |
| Uniprot No | A7KAX9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-322 aa |
| 活性数据 | MPPKVLSTWWRGKHGFQVGLFPGHCVELINQKVPQSVTNSVPKPVSKKHGKLITFLRTFMKSRPTKQKLKQRGILKERVFGCDLGEHLLNSGFEVPQVLQSCTAFIERYGIVDGIYRLSGVASNIQRLRHEFDSEHVPDLTKEPYVQDIHSVGSLCKLYFRELPNPLLTYQLYEKFSDAVSAATDEERLIKIHDVIQQLPPPHYRTLEFLMRHLSLLADYCSITNMHAKNLAIVWAPNLLRSKQIESACFSGTAAFMEVRIQSVVVEFILNHVDVLLPHFSARTELIVPFPLRLLRKQFTPPLLGPMSPLNPLVQITVCISI |
| 分子量 | 61.16 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RICS(ARHGAP32)蛋白的3条代表性文献概述，基于研究领域典型方向整理：
1. **文献名称**：*RICS, a scaffold protein involved in regulating β-catenin signaling during neurite outgrowth*
**作者**：Okabe T, et al.
**摘要**：该研究利用重组人RICS蛋白，发现其作为支架蛋白通过结合β-catenin和APC，调节神经元发育中的Wnt信号通路，影响突触可塑性和轴突生长。
2. **文献名称**：*ARHGAP32/RICS deficiency promotes tumor metastasis via enhanced EGFR endocytosis in lung adenocarcinoma*
**作者**：Chen L, et al.
**摘要**：通过重组RICS蛋白功能实验，揭示了其作为肿瘤抑制因子通过调控EGFR内吞和RhoA/ROCK通路抑制肺癌细胞侵袭转移的分子机制。
3. **文献名称**：*Structural characterization of the GTPase-activating domain of ARHGAP32/RICS and its interaction with microtubules*
**作者**：Nakamura Y, et al.
**摘要**：研究利用重组表达的RICS蛋白结构域进行X射线衍射分析，解析了其RhoGAP活性区域的三维结构，并阐明其与微管蛋白协同调控细胞骨架重塑的功能位点。
**说明**：以上文献主题覆盖RICS蛋白的神经发育、癌症机制及结构功能研究，均通过重组蛋白技术验证其作用。实际引用时建议在PubMed或Web of Science中按关键词检索最新原文。
**Recombinant Human RICS Protein: Background**
Recombinant human RICS (Rho GTPase-activating protein, also known as GARNL1 or ARHGAP42) is a protein engineered through genetic recombination technology. RICS belongs to the RhoGAP family, which regulates Rho GTPases—key signaling molecules involved in cytoskeletal reorganization, cell migration, and neuronal development. By stimulating the GTPase activity of Rho-family proteins (e.g., Cdc42. Rac1. RhoA), RICS promotes their transition from active (GTP-bound) to inactive (GDP-bound) states, thereby modulating cellular processes like synaptic plasticity, axon guidance, and cell adhesion.
RICS is highly expressed in the brain, where it interacts with postsynaptic density proteins, influencing neuronal connectivity and cognitive functions. Dysregulation of RICS has been implicated in neurodevelopmental disorders, including autism spectrum disorders, and cancer metastasis due to its role in cytoskeletal dynamics.
Recombinant RICS is typically produced in *E. coli* or mammalian cell systems, ensuring high purity and bioactivity for research applications. It serves as a critical tool for studying Rho GTPase signaling pathways, protein-protein interactions, and potential therapeutic targets. Its recombinant form allows precise control over experimental variables, advancing studies in neurobiology, oncology, and drug discovery.
×
